HIF-1α基因转染骨髓间充质干细胞治疗脊髓缺血-再灌注损伤早期超氧化物歧化酶活性和丙二醛含量的变化  

Effects of implantation bone mesenchymal stem cells modified by HIF-1α gene on SOD activity and MDA content changements after spinal cord ischemic reperfusion injury

作　　者：田丽颖[1] 吕苗苗[1] 张玉明[1] 王君[1] 吕海港[1] 张振[1] 蔡承魁[1] 高昌俊[1] 柴伟[1] 孙绪德[1] 

机构地区：[1]第四军医大学唐都医院麻醉科,西安市710038

出　　处：《临床麻醉学杂志》2013年第3期279-281,共3页Journal of Clinical Anesthesiology

基　　金：军队“十一五”科技攻关项目(编号:2009G167)

摘　　要：目的探讨低氧诱导因子-1α(HIF-1α)基因转染的骨髓间充质干细胞(BMSCs)在脊髓缺血-再灌注损伤(SCII)中的治疗作用及对超氧化物歧化酶(SOD)、丙二醛(MDA)的影响。方法采用腹主动脉肾动脉下方夹闭法造成大鼠SCII模型。55只成年SD大鼠随机分为三组:假手术组(Sham组,n=5)只暴露腹主动脉;SC组(n=25)SCII3h后髓内注射5μl生理盐水;HIF-1α基因修饰的BM-SCs组(HB组,n=25)SCII3h后髓内微量注射5μl含1×106个/ulHIF-1α-BMSCs的悬液。SC组和HB组连续检测治疗后(sham组于假手术后)2、6、12、18、24h脊髓组织内SOD活性和MDA含量的变化。结果与Sham组比较,治疗后2、6、12、18hSC组和HB组SOD活性明显降低(P<0.05);治疗后2、6、12、18、24hSC组和HB组MDA含量明显升高(P<0.05)。与SC组比较,治疗6、12、18hHB组SOD活性明显升高,MDA含量明显降低(P<0.05)。结论 HIF-1α基因联合BMSCs移植治疗SCII,可通过减轻组织缺氧,减少自由基的产生,保护脊髓组织,促进神经功能的恢复。Objective To investigate the changements of superoxide dismutase （SOD） and malondialdehyde （MDA） after spinal cord ischemia-reperfusion injury （SCII）, and the protection effects by injecting hone marrow mesenchyrnal stem cells （BMSCs） which modified by HIF-1α gene after SCII. Methods Abdo minal aortic corss-clamping was performed to get the spinal cord isehemia reperfusion injury model. 55 adult Sprague-Dawley（SD）rats were divided into 3 groups randomly. They are group Sham （n=5）, group SC（n=25） and HIF-1α-BMSCs group（group HB,n=25）. Three hours after reperfusion, we injected 5 ul cells which containing 1 × 10^6/ul HIF-1α-BMSCs into injuried spinal canal in group HB. The activities of SOD and the contents of MDA in spinal cord were measured 2, 6, 12, 18 and 24 h after the transplanted cells injection. Results The content of MDA increased immediately after reperfusion and reached the peak 6 h after injection. Then it started decreasing; the activities of SOD decreased significantly after injection and reached the lowest level 6 h after injection. Then it started ascending. In group HB, MI）A rose slowly and the peak value was lower; while SOD activity was bigger and peak value increased compared with those in group SC. Conclusion BMSCs modified by HIF-I~ gene transplantation in the treatment of spinal cord ischemia- reperfusion injury can attenuate tissue hypoxia, reduce the production of free radicals, protect spinal cord tissues and stimulate nerve function. It may provide a new idea and method for spinal cord ischemia-reperfusion injury therapy.

关 键 词：脊髓缺血-再灌注损伤 骨髓间充质干细胞 超氧化物歧化酶 丙二醛 

分 类 号：R614[医药卫生—麻醉学]