药物流产蜕膜中胰岛素样生长因子-II的基因表达  

Studies on Expression of IGF-II Gene in Deciduas Derived from Medical Abortion Patients

作　　者：汪玉宝[1] 刘峻[1] 毛全福[1] 杜晓岩[1] 

机构地区：[1]上海市计划生育科学研究所,上海200032

出　　处：《生殖与避孕》2000年第4期209-213,共5页Reproduction and Contraception

摘　　要：胰岛素样生长因子 -II(IGF-II)是一种重要的生殖调节因子。本文比较了人工流产与药物流产蜕膜中 IGF-II基因表达水平。结果显示人工流产蜕膜中 IGF-II基因呈现普遍的高表达 ,其基因转录相对值为 1 .5 4± 0 .79,而药物流产的蜕膜中 IGF-II基因表达显著下降 ,其基因转录相对值为 0 .72± 0 .39,两者有显著性差异 (P<0 .0 5 )。结论 :IGF-II可能介导了孕激素对蜕膜的支持作用 。Objective To determine the effect of insulin like growth factor Ⅱ(IGF Ⅱ) upon the maintenance of decidua in early pregnancy and its relationship with progesterone, as well as its role in medical abortion.Materials & Methods Decidua tissue was obtained from 28 women who undergoing surgical abortion and 39 for medical abortion respectively at 5~7 weeks of gestation. The extracted total RNA was reversely transcripted and amplified by PCR with specific primers (IGF Ⅱ and β actin). The products were semi quantitated by MIAS 300 system and qualitatively analyzed by southern blotting.Results The expression of IGF Ⅱ gene in decidua from surgical abortion was significantly higher than that from medical abortion (P<0.05). The average IGF Ⅱ gene transcription values were 1.54±0.79 and 0.72±0.39 respectively. The results of southern blotting proved qualitatively that the RT PCR products were IGF Ⅱ cDNA.Conclusion IGF Ⅱ plays a role in the maintenance of decidua in early pregnancy. It may act as a mediator of progestin. It's also involved in the molecular mechanism of mifepristone.

关 键 词：胰岛素样生长因子-Ⅱ 蜕膜 人工流产 药物流产 

分 类 号：R169.4[医药卫生—公共卫生与预防医学]