基于血浆药理学的鳖甲超微粉抗肝纤维化作用研究  

Studies of Anti-hepatic Fibrosis of Different Particle Size Trionyx Sinensis Wiegmann Based on Plasma Pharmacology

作　　者：徐平华[1,2] 靳世英[1,3] 李寅超[1,2] 迟淑萍[1] 徐和[1,3] 韩晋[1] 袁海龙[1] 

机构地区：[1]解放军302医院,北京100039 [2]郑州大学,河南郑州450001 [3]成都中医药大学,四川成都611730

出　　处：《解放军药学学报》2013年第1期9-12,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家科技重大专项;No.2011ZX09201

摘　　要：目的用血浆药理学方法,体外观察鳖甲超微粉含药血浆抗肝纤维化作用。方法采用体外肝纤维化细胞模型,以大鼠含药血浆对HSC-T6细胞增殖的影响为指标,采用效应动力学方法,确定大鼠给药方案和采血时间,并优选体系中血浆添加量。制备鳖甲超微粉和普通粉含药血浆,MTT法检测含药血浆对HSC-T6细胞增殖的影响,ELISA法测定含药血浆对HSC-T6细胞胶原分泌的影响。结果鳖甲超微粉和普通粉含药血浆对HSC-T6细胞增殖和Ⅰ型胶原合成均有显著抑制作用(P<0.01),超微粉组的抑制作用强于普通粉组,且有显著性差异(P<0.01)。结论鳖甲超微粉碎后可提高其抗肝纤维化疗效。Objective To investigate the effect of plasma-containing Trionyx sinensis Wiegmann uhrafine suspension on proliferation and type I collagen synthesis of HSC-T6 cells in culture based on the plasma pharmaco- logical method. Methods An in vitro HSC-T6 cell model was establishe to evaluate the level of hepatic fibrosis, and pharmacodynamics method was used to screen the method for preparing plasma-containing active ingredients and suitable concentration in the culture system. The amount of type I collagen secretion was measured by the method of ELISA. Results Plasma samples from ultrafine and ordinary fine suspension could obviously inhibit the proliferation and the secretion of type I collagen of HSC-T6 cells, and the activities of the former were significantly better（ P 〈 0.01 ）. Conclusion Micronization can improve the effect of Trionyx sinensis Wiegmann on anti-hepatic fibrosis.

关 键 词：鳖甲 超微粉 血浆药理学 HSC-T6细胞 

分 类 号：R965.2[医药卫生—药理学]