新型荧光探针罗丹明肉桂酰胺的合成及其用于荧光分光光度法测定汞  

Synthesis of Rhodamine Cinnamide and Its Use as a New Fluorescence Probe in Fluorospectrophotometric Determination of Mercury

作　　者：曹东磊[1] 颜范勇[1] 王猛[1] 温景云[1] 李雪芳[1] 付洋[1] 陈莉[1] 

机构地区：[1]天津工业大学中空纤维膜材料与膜过程省部共建国家重点实验室培育基地,天津300387

出　　处：《理化检验（化学分册）》2013年第2期138-141,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金项目(50973084);高等学校科学技术发展基金(20071214)

摘　　要：以罗丹明B和肉桂酸为主要反应物,制备了新型荧光探针罗丹明肉桂酰胺,并采用红外光谱、核磁和质谱对荧光探针的结构进行了表征。在含30%(φ)乙醇的pH 7.0 HEPES缓冲溶液中,荧光探针与Hg^(2+)形成1比1的络合物,通过测定在发射波长584 nm处的荧光强度,与汞的浓度在1.0×10^(-6)mol·L^(-1)以内呈线性关系,方法的检出限(3s)为7.4×10^(-9)mol·L^(-1)。试验结果表明:该荧光探针对Hg^(2+)具有很好的选择性,不受其他金属离子的干扰。A new fluorescence probe, rhodamine cinnamide, was synthesized with rhodamine and cinnamic acid; and structure of the probe was characterized by IR, NMR and MS. In a HEPES buffer solution of pH 7. 0 containing 30% （φ） ethanol, a complex with molar ratio of 1 to 1 was formed by the reaction of the probe with Hg^2＋. Linear relationship between values of fluorescence intensity and concentration of Hg^2＋ was found in the range within ×10^-6mol·L^-1 measured at the wavelength of 584 nm （λcm）, with limit of detection of ×10^-9mol·L^-1. It was found that this fluorescence probe exhibits highly selective toward Hg^2＋ , and the determination of H^2＋ was not interfered by other metal ions.

关 键 词：罗丹明肉桂酰胺 汞 荧光探针 

分 类 号：O657.3[理学—分析化学]