胶束增敏催化分光光度法测定火腿肠中痕量亚硝酸根  

Micelle-Sensitized Catalytic Spectrophotometric Determination of Trace Amount of Nitrite in Sausage

作　　者：杨风霞[1] 荆瑞俊[1] 王婷[1] 

机构地区：[1]河南科技学院化学化工学院,新乡453003

出　　处：《理化检验（化学分册）》2013年第2期146-148,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：河南省教育厅自然科学研究计划主要项目(2008B430007)

摘　　要：在硫酸介质和表面活性剂十二烷基硫酸钠(SDS)存在下,亚硝酸根对溴酸钾氧化亚甲基蓝的褪色反应具有催化作用,并且由于添加了SDS,体系的灵敏度明显提高,据此提出了测定痕量亚硝酸根的方法。亚硝酸根的质量浓度在0.02～1.0 mg·L^(-1)范围内与△A呈线性关系,方法的检出限(3s/k)为0.074 mg·L^(-1)。方法用于火腿肠中痕量亚硝酸根的测定,并做回收试验,测得回收率在98.8%～103%之间,测定值的相对标准偏差(n=5)在1.9%～2.4%之间。In a H2SO4 medium and in the presence of SDS, color-fading of methylene blue by oxidation with KBrO3 was catalyzed by NO^2-, and the sensitivity of the color-fading reaction was found higher than that of the reaction in the absence of SDS. Based on these facts, a micelle-sensitized catalytic spectrophotometric method for determination of trace amount of nitrite was proposed. Linear relationship between the magnitude of decrease in absorbance （△A） and mass concentration of nitrite was obtained in the range of 0. 02-1.0 mg·L^-1, with detection limit （3s/k） of 0. 074 mg·L^-1. In the determination of trace amount of nitrite in sausage sample by the proposed method, values of recovery found by standard addition method were in the range from 98. 8% to 103%, with values of RSD＇s （n=5） in the range of 1.9%-2.4%.

关 键 词：胶束增敏 催化分光光度法 溴酸钾 亚甲基蓝 亚硝酸根 

分 类 号：O657.32[理学—分析化学]