流动注射-分光光度法测定药物中盐酸吡硫醇含量  

FI-Spectrophotometric Determination of Pyritinol Hydrochloride

作　　者：过治军[1,2] 李华[3] 李景原[1] 

机构地区：[1]河南师范大学生命科学学院,新乡453007 [2]河南省高校道地中药材保育及利用工程技术研究中心,新乡453007 [3]新乡学院化学与化工学院,新乡453007

出　　处：《理化检验（化学分册）》2013年第2期202-204,207,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金项目(30970696)

摘　　要：在pH 3.85乙酸盐缓冲溶液中,盐酸吡硫醇可将Fe(Ⅲ)还原为Fe(Ⅱ),邻菲啰啉能与生成的Fe(Ⅱ)显色,在波长508 nm处有最大吸收峰,据此提出了一种停留流动注射-分光光度法间接测定盐酸吡硫醇含量的方法。盐酸吡硫醇的质量浓度在8.00～500 mg·L^(-1)范围内与其ΔA呈线性关系,方法的检出限(3s/k)为1.54 mg·L^(-1)。方法用于盐酸吡硫醇药物中盐酸吡硫醇的测定,方法的回收率在99.6%～100%之间,测定值的相对标准偏差(n=6)小于2%。In an acetate buffer medium of pH 3.85, Fe（ Ⅲ ） was reduced by pyritinol hydrochloride to give Fe（ Ⅱ ）, and a colored complex was formed by its reaction with ο-phenanthroline, having its absorption maximum at the wavelength of 508 nm. Based on these facts, the determination of pyritinol hydrochloride by stopped flow injection （FI）-spectrophotometry was proposed. Linear relationship between values of △A and mass concentration of pyritinol hydrochloride was kept in the range of 8. 00-500mg·L^-1 and detection limit （3s/k） of the method found was 1.54 mg·L^-1. The proposed method was used in the determination of pyritinol hydrochloride in its drugs, and values of recovery found were in the range of 99.6%- 100% with RSD （n=6） less than 2%.

关 键 词：流动注射 分光光度法 盐酸吡硫醇 邻菲啰啉 

分 类 号：O657.32[理学—分析化学]