脊髓损伤伴骨折大鼠血清中降钙素基因相关肽的变化  

Changes of calcitonin gene-related peptide in serum after spinal cord injury in rats

作　　者：姚建华[1] 时述山[1] 李亚非[1] 张立仁[1] 李增洲[1] 梁毅[1] 

机构地区：[1]北京军区总医院骨科,100700

出　　处：《中华创伤杂志》2000年第9期538-540,共3页Chinese Journal of Trauma

基　　金：国家自然科学基金!资助项目 (39770 745 )

摘　　要：目的　研究脊髓损伤伴骨折大鼠血清中降钙素基因相关肽 (CGRP)的变化。方法　110只雌性Wistar大鼠随机分成 3组 ,T10～ 11段脊髓横断 +右胫骨骨折组和单纯右胫骨骨折组各 5 0只 ,正常对照组 10只。正常对照组于 2 8d一批处死 ,其他两组于伤后 3,7,14,2 1,2 8d分批处死 ,摄X线片行骨痂评分 ,采用缓冲液法测定血清碱性磷酸酶 (ALP) ,放免法测定血清CGRP。　结果　脊髓损伤组伤后 3,7d ,ALP均显著升高 (P <0 .0 1) ;脊髓损伤组 2 1,2 8d ,骨痂X线评分高 ,骨痂量多 ;脊髓损伤组早期血清中CGRP显著升高 (P <0 .0 1) ,7d时达正常的 3倍以上。　结论　脊髓损伤后早期成骨能力增强 ,可能受血清中CGRP调节。Objective To study the changes of calcitonin gene-related peptide in serum after spinal cord injury with fracture in rats. Methods One hundred and ten female Wistar rats were used and divided into 3 groups: the spinal cord transection+tibial fracture group (50 rats), the tibial fracture group (50 rats),and normal control group (10 rats). The rats in normal control group were sacrificed at 28 d. The rats in other two groups were sacrificed in batches at 3,7,14,21 and 28 d after operation. The fractured tibias were taken X-ray films to evaluate the score of callus. The activities of alkaline phosphatase (ALP)in serum of rats were measured by AMP buffer method. Calcitonin gene related protein (CGRP) in serum of rats was measured by radioimmunoassay. Results Activities of ALP in serum of spinal cord injury group were higher than those in tibial fracture group at 3 d and 7 d ( P <0.01). According to X-ray films, the score of callus in spinal cord injury group was higher than that in tibial fracture group at 21 d and 28 d. The quantity of CGRP in serum of spinal cord injury group was higher than that in tibial fracture group at 3 d and 7 d ( P <0.01) and were three times higher than that in normal rats at 7 d. Conclusions Callus formation which may be regulated by CGRP in serum is enhanced at early stage after spinal cord injury in rats.

关 键 词：脊髓损伤 骨折 降钙素基因相关肽 放射免疫测定 

分 类 号：R651.2[医药卫生—外科学] R683[医药卫生—临床医学]