大鼠脑干损伤早期突触素的免疫组织化学改变  

Immunohistochemical changes of synaptophysin at early stage following brainstem injury in rats

作　　者：邓平[1] 徐小虎[2] 祝家镇[3] 宋一璇[3] 

机构地区：[1]广州医学院神经科学研究所,510182 [2]汕头大学医学院法医研究所 [3]中山医科大学法医学系

出　　处：《中华创伤杂志》2000年第9期541-543,共3页Chinese Journal of Trauma

基　　金：国家自然科学基金!资助项目 (39470 76 6 )

摘　　要：目的　探讨突触素在脑干损伤早期的改变及意义。　方法　针刺法造成大鼠脑干损伤 ,用免疫组织化学和计算机图像分析技术定量观察脑干中突触素在伤后不同时间的改变。　结果　脑干损伤部位的突触素免疫阳性物在伤后 3～ 6h显著减少 ,其后恢复正常 ,持续至伤后2 4h。　结论　脑干组织中的突触前成分在损伤早期即有改变 ,有可能影响神经递质的释放。Objective To study the significance of the changes of synaptophysin at early stage following brainstem injury. Methods After stabbing in rat's brainstem with a needle, the changes of synaptophysin in the brainstem at different time after injury were detected quantitatively with immunohistochemistry and computerized image analysis. Results The synaptophysin positivity in the injured areas of brainstem remarkably decreased at 3～6 h after injury, then returned to normal levels up to 24 h after injury. Conclusions The presynaptic element in brainstem is altered at early stage of injury, which may interfere with the release of neurotransmitter.

关 键 词：脑干损伤 突触素 免疫组织化学 

分 类 号：R651.1[医药卫生—外科学]