拮抗油菜菌核病菌的链霉菌分离筛选与鉴定  

Identification of Streptomyces antagonizing oilseed rape pathogen Sclerotinia sclerotiorum

作　　者：胡磊[1] 牛世全[1] 景彩虹[1] 达文燕[1] 朱学泰[1] 韩建山[1] 程晓[1] 张爱梅[1] 

机构地区：[1]西北师范大学生命科学学院甘肃兰州,730070

出　　处：《中国油料作物学报》2013年第1期69-73,共5页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31260134;30960078);2009年度人事部留学人员科技活动择优项目

摘　　要：从甘肃省河西走廊改良后的盐碱地分离链霉菌用于油菜菌核病菌的拮抗研究,并对拮抗菌进行鉴定。分离得到10株不同菌落形态的链霉菌,其中有2菌株对油菜菌核病有拮抗作用,编号Ⅳ22-3-3和Ⅳ22-3-12。与油菜菌核病进行对峙培养结果发现,Ⅳ22-3-3和Ⅳ22-3-12抑菌圈直径分别为1.2和0.9cm;离体叶菌丝接种试验表明,Ⅳ22-3-3和Ⅳ22-3-12对油菜菌核病菌的防治效果分别达63.5%和49.1%;Ⅳ22-3-12能在油菜菌核上定殖并寄生分解菌核,同对照相比菌核萌发率下降18.2%;通过形态培养特征、生理生化特征和16SrDNA鉴定表明,Ⅳ22-3-3为产水链霉菌(Streptomyces hydrogenans),Ⅳ22-3-12为球孢链霉菌(Streptomyces globisporus)。Different Streptomyces strains were isolated from saline - alkali soils of Hexi Corridor in Gansu Province. Among them, two strains named IV22-3-3 and 1V22-3-12 were found to be antagonists inhibiting Sclerotinia sclerotiorum isolated from oilseed rape. Detached leaf inoculation tests showed that the two strains had preventing effects of 63.5% and 49.1% respectively. Moreover, IV22-3-12 could colonize on the sclerotia and inhibit the germination of the pathogen. Compared to physiological saline （control）, IV22-3-12 decreased 18.2% of the sclerotia germination rate. Morphological and physiological-biochemical characteristics as well as phylogenetic analysis based on 16S rRNA gene sequence supported strain 1V22-3-3 to be Streptomyces hydogenans, and strain IV22-3-12 to be Streptomyces globisporus.

关 键 词：油菜 核盘菌 菌核 盐碱地 链霉菌 生物防治 

分 类 号：S565.406[农业科学—作物学]