病毒白细胞介素6对甲基转移酶1表达的影响  

Effects of interleukin-6 on the expression of DNA methyltransferase1

作　　者：徐建 许雨乔 潘世扬 彭姗姗 黄蕾 孙瑞红 

机构地区：[1]南京医科大学第一附属医院检验学部,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2013年第2期160-163,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助(30901262);江苏省自然科学基金(BK2009440);江苏省实验诊断学重点实验室基金(XK201114);江苏省高等学校大学生实践创新训练计划项目(ky102j201205);江苏高校优势学科建设工程基金

摘　　要：目的:观察病毒白细胞介素6(viral interleukin-6,vIL-6)对甲基转移酶1(DNA methyltransferase 1,DNMT1)表达的影响。方法:vIL-6真核表达质粒转染293T细胞,荧光定量RT-PCR和Western blot法检测DNMT1表达的变化,比色法检测DNMT1的活性。结果:荧光定量RT-PCR和Western blot均表明vIL-6可显著促进DNMT1的表达,且vIL-6能上调DNMT1的活性。结论:vIL-6能诱导DNMT1的表达,可能是卡波济肉瘤相关疱疹病毒(KSHV)致DNA甲基化异常,促进肿瘤发生发展的分子机制之一。Objective：To observe the effect of viral interleukin-6 （vIL-6） on the expression of DNA methyltransferase 1（DNMT1）. Methods：The eukaryotie expression plasmid carrying vIL-6 gene was transferred into 293T cells. The expression of DNMT1 was examined by quantitative RT-PCR and Western blot. DNMT1 activity was analyzed by colorimetly. Results：Quantitative RT-PCR and Western blot demonstrated that vIL-6 increased the expression of DNMT1. Furthermore,vIL-6 upregulated the activity of DNMT1. Conclusion：vIL-6 can induce the expression of DNMT1. It may be one of the molecular mechanisms of Kaposi＇s sarcoma associated herpesvirus（KSHV）-induced DNA methylation abnormalities and oncogenesis.

关 键 词：vIL-6 甲基转移酶1 卡波济肉瘤相关疱疹病毒 

分 类 号：Q786[生物学—分子生物学]