人类血小板抗原1～4系统基因分型  

Genotyping of Human Platelet Antigen Systems from 1 Through 4

作　　者：陆震宇[1] 刘达庄[1] 包于勤[1] 朱俊[1] 朱自严[1] 唐瑞峰[1] 

机构地区：[1]上海市血液中心血型参比研究室

出　　处：《中国输血杂志》2000年第3期146-148,共3页Chinese Journal of Blood Transfusion

基　　金：卫生部科研基金!资助 (编号 :982 2 75 )

摘　　要：目的 :建立人类血小板抗原 1～ 4系统序列特异性引物 (PCR SSP)分型方法。方法 :合成 14条引物 ,采用PCR SSP方法对 2 5名健康献血者的HPA 1～ 4系统进行基因分型 ;分型结果与采用等位基因特异性寡核苷酸点杂交 (PCR ASO)获得的结果进行比较。结果 :以PCR SSP方法对HPA 4个系统进行分型均取得了明确、满意的结果 ,且与PCR ASO方法获得的结果完全一致。结论 :人类血小板抗原PCR SSP基因分型方法具有简便、快速、准确等优点 ,具有广泛的应用前景。Objective:To set up the method of genotypeing the human platelet antigen(HPA)systems from 1 through 4 by sequence specific primers(PCR SSP).Methods:14 primers were synthesized so that the human platelet antigen systems from 1 through 4 in 25 healthy blood donors could be genotyped by PCR SSP method.The reusults of genotyping by PCR SSP were compared with those obtained by allele specific oligonucleotide hybridization(PCR ASO) Results:The results of genotyping the 4 HPA systems by PCR SSP were definite,satisfied and in complete agreement with those obtained by PCR ASO method.Conclusion: The PCR SSP method of genotyping the human platelet antigen has the advantages,such as simplicity,rapidity,accuracy and so on,opening up the broad prospects for its further application.

关 键 词：PCR-SSP 人类血小板抗原 基因分型 

分 类 号：R392.11[医药卫生—免疫学]