液相色谱-电喷雾离子阱质谱联用测定尿中γ-羟基丁酸  

Determination of Gamma-hydroxybutyric Acid in Urine by Liquid Chromatography-mass Spectrometry

作　　者：陈学国[1] 马生祥[1] 马玄中 党斌[1] 刘迟[1] 

机构地区：[1]中国刑警学院法化学系,辽宁沈阳110854

出　　处：《广东化工》2013年第5期17-18,共2页Guangdong Chemical Industry

基　　金：公安部应用创新项目(2010YYCXLNST012);辽宁省自然科学基金项目(No.201102246);上海市法医学重点实验室项目(No.KF1102)

摘　　要：文章建立了尿中γ-羟基丁酸液相色谱-电喷雾离子阱质谱分析方法。尿样经甲醇稀释后涡旋、离心,上清液过膜后直接进行液相色谱-电喷雾离子阱质谱联用分析。结果表明：尿中γ-羟基丁酸在0.50~100.00μg/mL之间具有良好的线性关系,最低检出限为0.10μg/mL。A method of analysis of gamma-hydroxybutyric acid in urine was presented using liquid chromatography and ion trap electro-spray mass spectrometry. Urine samples were diluted with methanol, the mixtures were stirred with a vortex mixer and centrifuged at 2000×g for 5 min, and the supernatants were directly analyzed by liquid chromatography and ion trap electro-spray mass spectrometry after being filtered through a 0.45 μm membrane. The calibration curve showed good linearity in the range of0.50-100.0 μg/mL and the limit of detection was 0. 10 μg/mL, respectively.

关 键 词：法医毒物分析 Γ-羟基丁酸 液相色谱-质谱 尿 

分 类 号：O65[理学—分析化学]