大黄附子汤血清药物化学初步研究  

Preliminary study on serum pharmacochemistry of Dahuang Fuzi Decoction

作　　者：郭辉[1,2,3] 刘晓[1,2,3,4] 蔡皓[1,2,3] 李嬛[1,2,3] 吴丽[1,2,3] 蔡宝昌[1,2,3,4] 

机构地区：[1]南京中医药大学药学院,江苏南京210046 [2]南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210046 [3]南京中医药大学国家中医药管理局中药炮制标准重点研究室,江苏南京210046 [4]南京海昌中药集团有限公司,江苏南京210061

出　　处：《中草药》2013年第5期528-531,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金面上项目(81073022)

摘　　要：目的对大黄附子汤进行血清药物化学初步研究,通过分析入血成分探讨该复方发挥药效的物质基础。方法采用血清药物化学的研究方法,同时建立大黄附子汤及大鼠ig给予大黄附子汤后血清HPLC指纹图谱,通过分析比较分别给予大黄附子汤、单味生药所得血清样品,初步鉴定大黄附子汤在大鼠血清中的移行成分。结果大鼠ig给予大黄附子汤后,血清中发现18个入血成分,其中14个为代谢产物;4个为原型成分。结论大黄附子汤入血成分主要来自大黄和附子,复方血清药物化学研究为深入阐述大黄附子汤药效物质基础提供依据。Objective To carry out a preliminary study on serum parmacochemistry of Dahuang Fuzi Decoction （DFD） and to research the pharmacodynamic material basis through analyzing the constituents absorbed into blood. Methods HPLC fingerprints of DFD and serum of rats after DFD treatment were established with serum pharmacochemistry method. The serum samples collected after DFD and single crude drug treatments were compared, the constituents absorbed into the serum after DFD ig administration was determined. Results After the rats were ig administered with DFD, 18 transitional constituents to blood were detected, among which 14 were metabolites and 4 were prototype constituents. Conclusion After DFD treatment, the constituents absorbed into blood are mainly from Rhei Radix et Rhizoma and Aconiti Lateralis Radix Praeparata, and the serum pharmacochemistry signs could provide the evidence for the further research of the material basis of the decoction.

关 键 词：大黄附子汤 血清药物化学 入血成分 指纹图谱 物质基础 

分 类 号：R284.1[医药卫生—中药学]