HPLC-UV技术用于川木香煨制前后代谢物轮廓差异研究  

Differences of metabolic profiling of Vladimiriae Radix before and after simmer processing by HPLC-UV technology

作　　者：高飞[1] 傅超美[1] 胡慧玲[1] 王战国[2] 傅舒[1] 毛茜[1] 盛菲亚[1] 彭伟[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137 [2]四川大学生命科学院,四川成都610041

出　　处：《中草药》2013年第5期547-551,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金青年基金项目(81001638)

摘　　要：目的比较川木香煨制前后代谢物轮廓(metabolic profiling)差异,筛选出导致差异的特征性化学成分。方法经HPLC-UV分析获得川木香生品、煨品的色谱图数据集;采用主成分分析(principal component analysis,PCA)、偏最小二乘法-判别分析(partial least square-discriminant analysis,PLS-DA)、聚类分析(hierarchical cluster analysis,HCA)等多变量统计分析方法比较川木香生品、煨品的代谢物轮廓差异及筛选导致差异的特征性化学成分。结果川木香生品和煨品代谢物轮廓明显不同,导致差异的特征性化学成分有7个(包含木香烃内酯和去氢木香内酯)。结论从代谢物轮廓角度,川木香煨制前后具有显著差异。Objective To compare the metabolic profiling of Vladimiriae Radix （VR） before and after simmer processing and to screen out the characteristic constituents leading to some differences. Methods The chromatogram data sets of the processed and unprocessed VR were obtained by HPLC-UV analysis; The metabolic profiling differences of VR before and after processing were compared by principal component analysis, partial least square-discriminant analysis, and hierarchical cluster analysis, then the characteristic constituents were screened out. Results The metabolic profilings between processed and unprocessed VR were apparently different, and the data showed seven constituents among them mainly made the quality difference, including costunolide and dehydrocostus lactone. Conclusion The processed and unprocessed VR have the significant differences on the metabolic profiling.

关 键 词：代谢物轮廓 川木香 煨制 HPLC-UV 特征性化学成分 木香烃内酯 去氢木香内酯 主成分分析 偏最小二 乘法-判别分析 聚类分析 

分 类 号：R283.1[医药卫生—中药学] R286.02[医药卫生—中医学]