红景天苷壳聚糖纳米粒的制备及其体外释放性能研究  

Preparation and in vitro drug release properties of salidroside-chitosan nanoparticles

作　　者：何黎黎[1] 邓黎[2] 林芸竹[3] 

机构地区：[1]西南民族大学,四川成都610041 [2]四川大学华西药学院靶向药物重点实验室,四川成都610041 [3]四川大学华西第二医院,四川成都610041

出　　处：《中草药》2013年第5期552-556,共5页Chinese Traditional and Herbal Drugs

基　　金：中央高校基本科研业务费专项基金项目(10NZYZJ06)

摘　　要：目的以壳聚糖为载体制备红景天苷壳聚糖纳米粒(SA-CS-NPs),并考察其体外释药特性。方法采用溶剂扩散-离子交联法制备SA-CS-NPs,考察其粒径分布和形态,并对SA-CS-NPs的包封率、载药量及其体外释药特性进行研究。结果所制得的SA-CS-NPs呈球形或类球形,平均粒径为(247.5±23.8)nm(n=3),Zeta电位为(23.4±2.7)mV(n=3),多分散指数(PDI)为0.265±0.071(n=3);平均包封率为(70.15±1.60)%,平均载药量为(14.03±0.32)%(n=3);24 h累积释放率达85%以上。结论溶剂扩散-离子交联法制备SA-CS-NPs具有合适的粒径和包封率,并能达到缓释效果。Objective To prepare salidroside-chitosan nanoparticles （SA-CS-NPs） and to evaluate the properties of in vitro drug release. Methods SA-CS-NPs were firstly prepared by solvent diffusion-ionic erosslinking method. The particle size and polydispersity of SA-CS-NPs were determined and the morphology of nanoparticles was evaluated. The properties of encapsulation efficiency （EE）, load efficiency （LE）, and in vitro release of SA-CS-NPs were also evaluated using UPLC method. Results The nanoparticles were successfully prepared with the spherical shape or para-spherical shape. The mean particle size was （247.5 ± 23.8） nm with the polydispersity index （PDI） of 0.265 ± 0.071, and the Zeta potential was （23.4 ± 2.7） mV （n = 3）. The EE was （70.15 ±1.60）% and the LE was （14.03 ± 0.32）% （n = 3）. The cumulative release rate of SA-CS-NPs within 24 h was over 85%. Conclusion SA-CS-NPs prepared by solvent diffusion-ionic crosslinking method show appropriate particle size and EE, and could exhibit sustained release properties in vitro.

关 键 词：红景天苷 壳聚糖纳米粒 溶剂扩散-离子交联法 体外释药 缓释 

分 类 号：R283.6[医药卫生—中药学] R286.02[医药卫生—中医学]