生血丸对小鼠低剂量辐射损伤的预防与修复作用及机制  

Prevention and rehabilitation of Shengxue Pills on mice injured by low-dose radiation and their mechanisms

作　　者：严苏纯[1] 张晓乐[2] 王光普[1] 齐立聪 

机构地区：[1]天津医科大学总医院中医科,天津300052 [2]天津医科大学研究生院,天津300072 [3]天津市蓟县中医医院,天津300900

出　　处：《中草药》2013年第5期595-597,共3页Chinese Traditional and Herbal Drugs

基　　金：天津市中医药管理局中医中西医结合科研专项资金(11103)

摘　　要：目的探讨生血丸对低剂量辐射致小鼠肝、脾、骨髓细胞、外周血淋巴细胞损伤的预防与修复作用及机制。方法将小鼠随机分为5组:对照组、生血丸预防给药组(预防给药组)、生血丸治疗给药组(治疗给药组)、模型1组(对应治疗给药组)、模型2组(对应预防给药组)。ELISA法检测肝、脾细胞中羟自由基、超氧阴离子自由基、鸟氨酸脱羧酶(ODC)的活性。高倍镜下观察骨髓细胞及外周血淋巴细胞的微核率。结果与对照组相比,2个模型组小鼠肝细胞内羟自由基、超氧阴离子自由基的量显著增加;与各自模型组相比,治疗给药组、预防给药组上述指标显著减小。与对照组相比,2个模型组小鼠肝细胞内ODC活性显著增强,外周血淋巴细胞与骨髓细胞的微核率显著增加;与各自模型组相比,预防给药组和治疗给药组ODC活性显著减弱,外周血淋巴细胞与骨髓细胞的微核率显著降低。各组小鼠脾细胞中各指标间未见明显差异。结论生血丸能有效预防和改善小鼠肝细胞、外周血淋巴细胞和骨髓细胞由于低剂量辐射所造成的损伤。Objective To study the prevention and rehabilitation of Shengxue Pills on liver, spleen, bone marrow cells, peripheral blood lymphocyte of mice injured by low-dose radiation and their mechanisms. Methods Mice were randomly divided into five groups： control, Shengxue Pills prevention, Shengxue Pills rehabilitation, model 1 （corresponding to rehabilitation）, and model 2 （corresponding to prevention） groups. Using the ELISA method to detect the activity of hydroxyl radical, superoxide anion radical, and omithine decarboxylase （ODC） in liver and spleen cells. The bone marrow cells and peripheral lymphocyte micronucleus rate were observed under high power lens. Results Compared with control group, the amounts of hydroxyl radical and superoxide anion radical in the two model groups were obviously increased. Compared with the corresponding model groups, the above indexes in prevention group and rehabilitation group were significantly decreased. Compared with the control group, the activity of ODC in mice of the two model groups was obviously enhanced, but bone marrow cells and peripheral lymphocyte micronucleus rate were increased. Compared with the corresponding model groups, the activity of ODC in mice of prevention and rehabilitation groups was weakened, and bone marrow cells and peripheral lymphocyte micronucleus rate were decreased. But each index of spleen cells showed no significant difference. Conclusion Shengxue Pills could effectively prevent and rehabilitate the damage in liver cells, peripheral blood lymphocytes, and bone marrow cells of mice injured by low-dose radiation.

关 键 词：生血丸 低剂量辐射 辐射损伤 羟自由基 超氧阴离子 鸟氨酸脱羧酶 微核率 

分 类 号：R285.5[医药卫生—中药学] R979.6[医药卫生—中医学]