地茎段离体培养再生植株  

Plant regeneration in vitro from stem segments of Melastoma dodecandrum

作　　者：曹智[1] 

机构地区：[1]福建师范大学福清分校生物与化学工程系,福建福清350300

出　　处：《宁德师范学院学报（自然科学版）》2013年第1期36-39,共4页Journal of Ningde Normal University(Natural Science)

摘　　要：以地菍的茎作为外植体,研究了离体培养再生植株.试验结果表明:在配方为MS+6-BA1.5mg/L+NAA1.0mg/L固体培养基中诱导不定芽,诱导率高达76.6%;继代增殖用MS+6-BA0.25mg/L+NAA1.0mg/L较为理想,增殖系数为8.8,而生根培养基为MS+6-BA0.25mg/L+NAA1.0mg/L生根效果较好.The technique of in vitro culture production of Melastoma dodecandrum was developed from the explants taken from stem segments. The results showed that on the solid MS medium supplemented with 6-BA 1.5mg/L and NAA 1.0 mg/L for inducing the germination adventitious buds, the inducing rate was 76.6%. The solid MS medium with 6-BA 0.25mg/L and NAA 1.0mg/L were used for multiplication, the proliferation rate was over 8.8. It was the appropriate rooting culture medium of MS with 6-BA 0.25mg/L and NAA 1.0mg/L.

关 键 词：地菍 离体培养 再生植株 

分 类 号：S339.41[农业科学—作物遗传育种]