乙醛脱氢酶-1作为心肌干细胞有效标志物的实验研究  

Aldehyde dehydrogenase-1 as an effective marker for cardiac stem cells

作　　者：韦海珠[1] 胡琳洁[1] 梁冬[1] 

机构地区：[1]海南省农垦三亚医院,海南三亚572000

出　　处：《哈尔滨医科大学学报》2013年第1期28-30,34,共4页Journal of Harbin Medical University

基　　金：三亚市医疗卫生科技创新项目(YW1210);黑龙江省科技计划项目(GZ11C012)

摘　　要：目的研究乙醛脱氢酶-1(ALDH-1)是否可以作为分选心肌干细胞(CSC)的有效标志物。方法从裸鼠心脏分离培养细胞球,收集细胞球制成单细胞悬液,利用Aldefluor试剂结合流式细胞术来分选心脏球体细胞中的SSCloAldebr细胞(ALDH-1阳性细胞),通过增殖能力、克隆形成、表型分析及定向分化能力鉴定其干细胞(SC)的特性。结果裸鼠心脏细胞无血清培养可形成细胞球,球体细胞中可检测到ALDH-1阳性细胞的存在;ALDH-1阳性细胞具有高增殖性、高克隆形成率及定向分化的能力,具有SC的特性。结论裸鼠心脏中存在CSC;ALDH-1可以作为CSC有效的标志物。Objective To investigate whether acetaldehyde dehydrogenase-1 （ALDH-1） may be used as an effective marker for sorting of cardiac stem cells （CSC）. Methods Cells were separated from the heart of nude mice and cultured, and then collected to prepare single cell suspension. Utilizing Aldefluor reagent in conjunction with flow cytometry, SSC^lo Alde^br cells （ ALDH-1 positive cells） were sorted from collected cardiac cells. Characteristics of the cardiac stem ceils were identified by analyzing reproductive capacity, clone formation, phenotypes and oriented differentiation of the sorted cells. Results Through serum-free culture cardiac cells from the heart of nude mice formed heter-cell spheres. In spheroid cells ALDH-1 positive cells were found. ALDH-1 positive cells possessed characteristics of stem cells including high repro- t ductive capacity, high clone forming rate and ability for oriented differentiation. Conclusion Cardiac stem cells exist in the heart of nude mice, and ALDH-1 may be used as an effective marker for cardiac stem cells.

关 键 词：乙醛脱氢酶-1 心肌干细胞 流式分选 鉴定 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]