白藜芦醇对H_2O_2所致乳鼠心肌细胞损伤的保护机制研究  

Protective mechanism of resveratrol in H_2O_2 induced neonatal cardiomyocytes injury

作　　者：张莹[1] 沐晓芹[1,2] 梁海海[1] 路慧敏[1,3] 初霞[1,3] 朱久新[1] 

机构地区：[1]哈尔滨医科大学药理学教研室,黑龙江哈尔滨150081 [2]哈尔滨医科大学基因组中心,黑龙江哈尔滨150081 [3]哈尔滨医科大学营养与食品卫生学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2013年第1期43-45,共3页Journal of Harbin Medical University

基　　金：2010年黑龙江省教育厅科学技术研究面上项目(11551276)

摘　　要：目的研究白藜芦醇(Res)在H2O2所致乳鼠心肌细胞损伤中的保护机制。方法将原代培养的乳鼠心肌细胞分成3组:对照组,H2O2处理组和Res+H2O2处理组,采用MTT比色法检测细胞活性,应用Real Time-PCR技术检测miR-34a的表达水平。结果 H2O2对乳鼠心肌细胞具有损伤作用,Res可明显缓解H2O2对乳鼠心肌细胞的损伤作用,经过H2O2处理后乳鼠心肌细胞内miR-34a的表达水平显著升高,Res可明显降低乳鼠心肌细胞内miR-34a的表达水平。结论 Res通过下调miR-34a的表达水平来发挥对H2O2所致乳鼠心肌细胞损伤的保护作用。Objective To investigate the protective mechanism of resveratrol （Res） inH2O2induced neonatal cardiomyocytes injury. Methods Primary cultured neonatal cardiomyocytes were divided into 3 groups： control, H2O2 group and Res group. MTF assay was used to detect cell viability and real time PCR was used to detect miR-34a expression in each group. Results Res rescued neonatal cardiomyocytes injury caused by H2O2. Moreover, Res up-regulated the decreased miR-34a level induced by H2O2. Conclusion Res can rescue HaO2 induced neonatal cardiomyocytes injury by up-regulating miR-34a expression.

关 键 词：白藜芦醇 过氧化氢 心肌细胞 损伤 miR-34 

分 类 号：R966[医药卫生—药理学]