轮状病毒LLR株在两种不同细胞中病毒滴度的比较  

Comparison of titers of rotavirus LLR strain cultured in two kinds of cells

作　　者：林杰[1] 窦强[1] 刘溯[1] 魏至栋[1] 

机构地区：[1]兰州生物制品研究所有限责任公司,兰州730046

出　　处：《中国新药杂志》2013年第6期643-646,共4页Chinese Journal of New Drugs

摘　　要：目的:通过轮状病毒LLR株在牛肾细胞和Vero细胞中培养效果的比较,为轮状疫苗的生产筛选最佳的细胞基质和培养条件。方法:将轮状病毒LLR株按MOI 0.02分别接种牛肾细胞和Vero细胞,在相同培养条件下进行培养,每天观察两种细胞病变的情况,同时抽样检测病毒滴度,分析两种细胞对轮状病毒LLR株的敏感性。结果:牛肾细胞在感染轮状病毒LLR株后d 3病毒滴度达到最高,为6.8 lgCCID50.mL-1;而Vero细胞在感染轮状病毒LLR株后d 8病毒滴度达到最高,为7.3 lgCCID50.mL-1。轮状病毒LLR株在牛肾细胞和Vero细胞上均能达到满意的病毒表达量。结论:轮状病毒LLR株在Vero细胞中培养能够达到理想的病毒表达量,利用Vero细胞培养轮状病毒LLR株,有利于提高疫苗的产量和质量。Objective： To screen the optimal cells matrix and the culture conditions for the production of rotavirus vaccine by comparing the culture results of rotavirus LLR strain in bovine kidney cells and Vero cells. Methods： LLR strain was inoculated on bovine kidney ceils and Vero cells under the same culture conditions. The CPE of two kinds of ceils was observed at different times,while the virus titer was detected though sampling, and the sensitivity of two kinds of cells to LLR strain was analyzed. Results： The virus titers of rotavirus LLR strain grown in the two kinds of cells were different： the titer in bovine kidney cells reached the highest value as 6.8 lgCCID50·mL-1 after three days and that in Vero cells reached 7.3 lgCCID50·mL-1 after eight days. Conclusion：The rotavirus LLR strain shows the optimal expressions in Vero ceils, so this kind of cells can be used to culture rotavirus LLR strain and improve the yield and quality of vaccine.

关 键 词：轮状病毒LLR株 牛肾细胞 VERO细胞 病毒滴度 

分 类 号：R979.5[医药卫生—药品]