大鼠海洛因戒断、脱毒和复吸对胰岛B细胞及血糖浓度的影响  

Influence of heroin withdrawal, detoxication and relapse on islet B cells and blood sugar concentration in rat

作　　者：韩晶[1] 梁文妹[1] 洪艳[1] 夏白娟[1] 胡赟[1] 李一欣[1] 

机构地区：[1]贵阳医学院组织学与胚胎学教研室,贵阳550004

出　　处：《解剖学杂志》2013年第1期21-24,共4页Chinese Journal of Anatomy

基　　金：贵州省社会发展科技攻关计划基金（黔科合s字（2007）1048）

摘　　要：目的：探讨在大鼠海洛因戒断、脱毒治疗和复吸期间，胰岛B细胞发生的形态和功能变化以及血中葡萄糖浓度的改变。方法：正常雄性SD大鼠，随机分为正常对照组和实验组，实验组分别建立海洛因依赖戒断模型、美沙酮脱毒治疗模型和海洛因复吸模型。免疫组织化学显色、图像分析观察胰岛B细胞在戒断、脱毒和复吸期间的形态改变；放射免疫检测血清胰岛素水平的变化。血糖仪测各组空腹血糖水平的变化。结果：光镜下，戒断、脱毒和复吸期间胰岛B细胞免疫反应显色均变浅。放射免疫检测血中胰岛素水平在戒断期间尚未发生改变，但脱毒组与复吸组胰岛素浓度均低于正常组，复吸组较脱毒组浓度又稍有升高；戒断组血糖水平较正常对照组升高，脱毒组和复吸组均无明显变化。结论：海洛因戒断、脱毒和复吸期间，胰岛素水平先减少后增多；戒断时血糖升高，脱毒和复吸时恢复正常。表明胰岛B细胞通过改变合成和分泌胰岛素维持机体血糖内稳态。Objective： To investigate the morphological and functional changes of the islet B-cells and glucose level response to heroin withdrawal induced by naloxone, methadone detoxification treatment, and relapse. Methods： Male Sprague-Dawley rats were divided into a normal control group （NCG） and an experiment group （EG）. The EG was further divided into a heroin withdrawal group （HWG）, a methadone detoxication group （MDG）, and a heroin relapse group （HRG）. Pancreas was drawn from each group. Immunohistochemistry and image analysis were used to detect the expression of insulin. Radioimmunoassay （RIA） were used to measure insulin level in the blood. Fast glucose was tested by glucose meter. Results： Immunohistochemical staining results revealed that less insulin expressed in B cells of islets from heroin withdrawal, detoxification, and relapse ＇rats than that in control~ Compared with controls, the insulin levels were significantly decreased in detoxification and re- lapse rats~ Blood glucose was significantly increased response to heroin withdrawal induced by naloxone, and no differences were found during detoxification treatment and relapse compared to controls. Conclusion： During heroin abstinence, detoxification, and relapse, B cells participate in maintaining the body＇s blood sugar homeostasis via changing synthesis and secretion insulin.

关 键 词：胰岛素 血糖 海洛因 美沙酮 复吸 大鼠 

分 类 号：R163[医药卫生—公共卫生与预防医学]