阿托伐他汀对兔动脉粥样硬化的作用及其机制  

Effect of atorvastatin on atherosclerosis and its mechanism in rabbit

作　　者：陈齐虹[1] 顾水明[1] 郑宏超[1] 缪培智[1] 赵莉芳[1] 曹佳齐[1] 

机构地区：[1]上海市徐汇区中心医院心内科,上海200031

出　　处：《解剖学杂志》2013年第1期29-32,共4页Chinese Journal of Anatomy

摘　　要：目的：观察阿托伐他汀对动脉粥样硬化（AS）早期病变的作用。方法：雄性新西兰白兔随机分为正常对照组（正常饮食）、动脉粥样硬化组（高脂饮食）和阿托伐他汀组,10周后测定血清中血脂、肿瘤坏死因子-α（TNF-α）、C反应蛋白（CRP）及白介素-10（IL-10）水平,H-E染色测定胸主动脉内膜及中膜厚度,免疫组织化学法测定胸主动脉环氧化酶2（COX-2）、氧化低密度脂蛋白受体1（LOX-1）及α肌动蛋白（α-actin）的表达。结果：高脂饮食后兔血清总胆固醇（TC）、甘油三酯（TG）,低密度脂蛋白胆固醇（ox-LDL）浓度升高,胸主动脉形成典型脂质条纹。与动脉粥样硬化组相比,阿托伐他汀组血清血脂、ox-LDL、 TNF-α、 CRP水平降低,IL-10水平则增高。胸主动脉内膜厚度和内膜/中膜厚度比下降,主动脉COX-2、 LOX-1、 α-actin的表达降低。结论：阿托伐他汀通过抑制血管炎症反应及平滑肌细胞增殖、降脂等多环节抑制动脉粥样硬化斑块的早期形成。Objective： To observe the effect ofatorvastatin on the progression of atherosclerosis （AS）. Methods： The New Zealand male rabbits were divided randomly into 3 groups （n= 7）, including normal control group （normal diet）, AS group （high-cholesterol diet）, atouast group. All rabbits were sacrificed after 10 weeks. Blood was drawn for quantify the serum lipid levels and the levers of creactive protein （CRP）, tumor necrosis factor-α（TNF-α） and interleukin-10 （IL-10）. Thoracic aorta were obtained for hematoxylin-eosin （HE） staining and cycloxygenase; epoxidase-2 （COX-2）, lectin-like oxidized low density lipoprotein-1 （LOX-1） and α-acttn immmunohistochemistry assay. Results： In all cholesterol-fed rabbits, the level of serum TC, TG, LDL and α-LDL were increased remarkably. The typical fatty streaks on the aorta had been formed. Com- pared to AS group, atorvastatin could decrease serum lipid, atorvastatin also could decrease serum CRP, TNF-α level and in- crease serum IL-10 level significantly. Atorvastatin can downregulate the COX-2, LOX-1 and α-actin expression significantly as compared with AS group. Conclusion： Atorvastatin can suppress vascular inflammation, inhabit.smooth muscle proliferation and lower blood lipid, all these contributed to inhibit the formation of the early atheroselerotic lesion in cholesteral-fed rabbits.

关 键 词：动脉粥样硬化 阿托伐他汀 炎症因子 环氧化酶-2 人氧化低密度脂蛋白受体1 Α肌动蛋白 

分 类 号：R543.5[医药卫生—心血管疾病]