生长追赶宫内发育迟缓大鼠脂肪组织中巨噬细胞浸润及TNF-α/IL-6分泌水平  

Accumulation of macrophages in adipose tissue and secretion of TNF-α/IL-6 in intrauterine growth retarded rats with catch-up growth

作　　者：王群[1,2] 侯凌[1] 叶娟[1] 高金枝[1] 应艳琴[1] 罗小平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,湖北武汉430030 [2]武汉市儿童医院,湖北武汉430016

出　　处：《临床儿科杂志》2013年第3期247-249,共3页Journal of Clinical Pediatrics

基　　金：湖北省自然科学基金资助项目(No.2009CDB374)

摘　　要：目的研究生长追赶宫内发育迟缓(CG-IUGR)大鼠脂肪组织中巨噬细胞浸润及分泌炎症细胞因子水平改变,以探讨CG-IUGR大鼠脂肪组织炎症反应与胰岛素抵抗的关系。方法 SD大鼠怀孕期间全程给予正常孕鼠食量的30%以限制饮食,并通过增加仔鼠哺乳期营养摄入建立CG-IUGR大鼠模型。以8周龄雄性CG-IUGR仔鼠为研究对象,取附睾脂肪组织做石蜡切片,通过免疫组化染色鉴定脂肪组织中浸润的巨噬细胞。大鼠原代成熟脂肪细胞和巨噬细胞用Transwell系统行共培养,ELISA法测定细胞培养液上清中炎症因子IL-6和TNF-α表达水平。结果 8周龄CG-IUGR仔鼠体质量、体质指数均高于对照组(P均<0.01);脂肪组织中巨噬细胞浸润较对照组大鼠增多;细胞共培养体系培养液中IL-6、TNF-α水平较对照组增高(P均<0.01)。结论 CG-IUGR大鼠脂肪组织存在巨噬细胞浸润和慢性炎症反应,参与机体胰岛素抵抗的形成。Objective To investigate the changes of macrophages infiltration in the adipose tissue of intrauterine growth retarded rats with catch-up growth（CG-IUGR）,and to investigate the secretion of proinflammatory cytokines in order to explore the impact of inflammation in adipose tissue of CG-IUGR rats with insulin resistance.Methods Rats in food-restrict group were fed 30% of intake of food comsumed by control group during pregnant,and the newborns were fed more breast milk to ensure their catch-up growth.Adipose tissue was isolated from epididymal fat pad of 8 week-old rats and paraffin section was stained by immunohistochemistry.Primary cultured rat peritoneal macrophages were co-cultured with primary cultured rat adipocytes for 24 hours in transwell inserts.The levels of IL-6 and TNF-α in the supernatant of co-cultured cells were detected by ELISA.Results The weight and body mass index（BMI） of 8 week-old CG-IUGR rats were higher than those of controls（P0.01）.The accumulation of macrophages in adipose tissue of 8 week-old IUGR rats was more than that in control rats.The levels of proinflammatory cytokines IL-6 and TNF-α in the supernatant of co-cultured cells of CG-IUGR rats were higher when compared with the controls（both P0.01）.Conclusions Adipose tissue of CG-IUGR rats is characterized by macrophage infiltration and these macrophages are an important source of inflammation in adipose tissue.

关 键 词：宫内发育迟缓 脂肪细胞 炎症反应 胰岛素抵抗 大鼠 

分 类 号：R714.5[医药卫生—妇产科学]