转TaLEA基因小黑杨株系变异及生长稳定性分析  

Variation and growth adaptability analysis of transgenic Populus simonii×P. nigra lines carrying TaLEA gene

作　　者：李志新[1] 赵曦阳[1] 杨成君[1] 汪广宇 王福森[3] 张连飞 张连才[5] 刘桂丰[1] 姜静[1] 

机构地区：[1]东北林业大学林木遗传育种国家重点实验室 [2]黑龙江省大庆市绿化工程有限责任公司 [3]黑龙江省森林与环境科学研究院 [4]黑龙江省庆安国有林场管理局 [5]吉林省白城市林业科学研究院

出　　处：《北京林业大学学报》2013年第2期57-62,共6页Journal of Beijing Forestry University

基　　金："863"国家高技术研究发展计划项目(2011AA100201)

摘　　要：以6个地点栽培的11个转TaLEA基因小黑杨株系及1个对照株系为材料,对2年生株系的树高和地径进行调查分析。方差分析结果表明:树高、地径在不同地点间、不同株系间的差异达极显著水平(P<0.01),株系×地点间的交互作用达显著水平(P<0.05);不同地点12个小黑杨转基因株系2年生树高平均值变化范围为128～235cm,地径变化范围为13～24mm;不同地点参试株系树高和地径变异系数变化范围分别为19.84%～33.38%和24.21%～49.16%;重复力变化范围为0.497～0.952,表明相同地点不同株系树高和地径差异较大,但差异主要由遗传因素控制,有利于株系选择。采用Tai模型对各株系的遗传稳定性进行评价,其中XL11株系为不稳定株系,其他11个为稳定性较强的株系。根据各株系的稳定性参数和树高与环境的交互作用效应值,预测了各株系的适生地区,其中XL9、XL1、XL14等3个株系在6个试验地点的生长量大、稳定性强,是6个试验点推广的首选株系。A totlal of 12 Populus simonii ~ P. nigra lines （11 transgenic Populus lines and one control line） in 2 years old were used to study the phenotypic variation of tree height（H） and basic diameter（BD） in 6 different sites. The analysis of variance showed that there was a remarkable difference in H and BD among sites （P〈0.01）, lines （P〈0.01）, and sitesxlines （P〈0.05）. The average H and BD of transgenic poplar lines ranged in 128-235 cm and 13-24 mm in different sites respectively. The coefficients of H and BD of transgenic poplar lines changed in 19.84% -33.38% and 24.21% -49.16% and repeatabilities ranged in 0. 497-0. 952, indicating that the variation of poplar transgenic lines was strongly controlled by genetic factors,which were in favor of line selection. Tai model was introduced to analyse the genetic adaptability of poplar lines in different sites. The results showed that except line XLll, other lines grew steady in different sites. Based on the genetic adaptability parameter and the domino effect values of interaction between H and environment, the most adapted site of each line was predicted. Lines XL9, XL1 and XL14 are high biomass and strong stability, which are suitable generalized in 6 different sites.

关 键 词：LEA基因 小黑杨 多点试验 遗传稳定性 

分 类 号：S722.5[农业科学—林木遗传育种]