白头翁素对腹泻小鼠肠黏膜修复因子EGFR和TGFβ1的影响  

Effect of Anemonin on Intestinal Mucosa Repair Factors EGFR and TGFβ1 of Diarrheal Mice

作　　者：刘颖[1] 徐倩倩[1] 薛晓阳[1] 褚军[1] 肖静静[1] 陈立功[1] 董世山[1] 

机构地区：[1]河北农业大学动物医学院,河北省兽用生物制品工程技术研究中心,保定071001

出　　处：《畜牧兽医学报》2013年第3期475-480,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：河北省科技支撑计划项目(07220401D-01)

摘　　要：白头翁素是白头翁的主要成分,对腹泻具有显著的防治效果。本试验利用猪轮状病毒和大肠杆菌混合感染建立腹泻模型,通过免疫组织化学方法和RT-PCR方法研究白头翁素对腹泻小鼠肠黏膜修复因子EGFR和TGFβ1表达的影响,揭示白头翁素防治腹泻的肠黏膜修复机制。结果显示:小鼠腹泻初期,自愈组、治疗组和预防组黏膜修复因子EGFR和TGFβ1的蛋白表达量均有所升高,其中预防组较空白对照组差异显著(P<0.05),EGFR和TGFβ1的mRNA表达量较空白对照组也显著升高,且差异极显著(P<0.01)。腹泻后期,表达量逐渐下降直至正常。试验结果表明:白头翁素对轮状病毒和大肠杆菌混合感染性腹泻良好的预防效果可能与其调节黏膜修复因子EGFR和TGFβ1的表达有关,从而促进肠黏膜的修复。Anemonin is the main component of pulsatilla, it has conspicuous control efficiency on diarrhoea. The aim of this study was to clarify the intestinal mucosa repair mechanism of anemonin on diarrhoea. The experiment established the diarrhoea model by porcine rotavirus （PRV） and E. coli, immunohistochemistry and RT-PCR were used in the study. The results showed that. In the preliminary stage of diarrhoea, the protein of TGFβI1 and EGFR in self-healing group, treatment group and prevention group were all higher than that of control group, and the prevention group was significantly higher than that of control group （P〈0.05）. The expression of EGFR mRNA and TGFI31 mRNA in self-healing group, treatment group and prevention group were all significantly higher than that of control group （P〈0.01）. In the later stage of diarrhoea, the expression of EGFR and TGFβI gradually decreased until to the normal condition. The results suggeste that the mechanism of anemonin could significantly prevent diarrhoea caused by PRV and E. coli maybe has some relationships with regulating the expression of F.GFR and TGFβ1.

关 键 词：白头翁素 小鼠 腹泻 黏膜修复 表皮生长因子受体 转化生长因子Β1 

分 类 号：S853.74[农业科学—临床兽医学]