黄皮叶萃取物镇咳、祛痰及平喘作用研究  

Studies on Antibechic, Expectorant and Antiasthmatic Effects of Extracts from Clausena Lansium (Lour.) Skeels Leaves

作　　者：黄桂红[1] 邓航[1] 陈薇[1] 罗昱澜[1] 董晓敏[1] 罗力立[2] 

机构地区：[1]桂林医学院附属医院,541001 [2]桂林医学院药学院2008级

出　　处：《天津医药》2013年第3期234-237,共4页Tianjin Medical Journal

基　　金：广西自然科学基金项目(项目编号:2012GXNSFAA053079);广西中医药科技专项项目(项目编号:GZKZ10-124);桂林市科学研究与技术开发计划项目(项目编号:20120121-1-17)

摘　　要：目的研究黄皮叶不同溶剂萃取物的镇咳、祛痰、平喘作用。方法 (1)将60只小鼠随机分为正常组、枸橼酸喷托维林组、石油醚萃取物组、二氯甲烷萃取物组、乙酸乙酯萃取物组及水提物组,给予相应药物灌胃7d,用浓氨水引咳,观察小鼠咳嗽潜伏期及2min内咳嗽次数。(2)将另60只小鼠随机分为正常组、氯化铵组、石油醚萃取物组、二氯甲烷萃取物组、乙酸乙酯萃取物组及水提物组,灌胃相应药物7d后采用腹腔注射5%酚红溶液,观察小鼠气管酚红排泌量。(3)70小鼠随机分为正常组、模型组、地塞米松组、石油醚萃取物组、二氯甲烷萃取物组、乙酸乙酯萃取物组及水提物组,于第1、7、14天腹腔注射卵蛋白致敏液;第18～22天给予相应药物灌胃,并用卵蛋白溶液雾化激发,观察哮喘小鼠支气管肺泡灌洗液(BALF)中白细胞总数及细胞分类计数。结果 (1)与正常组比较,石油醚萃取物、二氯甲烷萃取物能延长小鼠咳嗽潜伏期并减少2min内咳嗽次数,差异有统计学意义(P<0.01)。(2)与正常组比较,二氯甲烷萃取物、乙酸乙酯萃取物及水提物能增加小鼠气管酚红排泌量,差异有统计学意义(P<0.01)。(3)与模型组比较,石油醚萃取物能减少哮喘小鼠BALF液中白细胞总数、嗜酸性粒细胞比例及中性粒细胞比例,差异有统计学意义(P<0.01)。结论黄皮叶萃取物具有明显的镇咳、祛痰及平喘作用。Objective To study the antibechic, expectorant and antiasthmatic actions of Clausena Lansium （Lour.） Skeels （CLLSL） leaf extracts. Methods （ 1 ） Sixty mice were randomly divided into six groups： normal control group, pent- oxyverine group, petroleum ether extraction group, dichloromethane extraction group, ethyl acetate extraction group and liquid extraction group. After corresponding drug administration for seven days, cough models were induced by concentrated ammo- nia in mice. The latent phase and the cough frequency per two minutes were recorded in mice. （2）Another sixty mice were randomly divided into six groups： normal control group, ammonium chloride group, petroleum ether extraction group, dichloro- methane extraction group, ethyl acetate extraction group and liquid extraction group. After corresponding drug administration for seven days, the concentration of tracheal phenolsulfonphthalein was detected by 5% phenolsulfonphthalein solution intra- peritoneal injection. （3）Seventy mice were randomly divided into seven groups： normal control group, model group, dexameth- asone group, petroleum ether extraction group, dichloromethane extraction group, ethyl acetate extraction group and liquid ex- traction group. The asthmatic mouse models were established by ovalbumin （OVA）intraperitoneal injection at the 1＇h, 7＇h and 14＇h day, and atomization inhalation was given from 18-22 days. The asthmatic mice were treated with corresponding medi- cines respectively before atomization inhalation. The total leukocyte number and different cell counts in bronchoalveolar la- vage fluid （BALF） were observed. Results （1） Compared with normal control group, the latent phase was significantly longer and the cough frequency per two minutes was significantly reduced in petroleum ether extraction and dichloromethane extrac- tion groups （P〈 0.01）. （2） Compared with normal control group, dichloromethane extraction, ethyl acetate extraction and liq- uid extraction could significant

关 键 词：镇咳药 祛痰平喘 酚磺酞 白细胞计数 粒细胞 疾病模型 动物 黄皮叶 

分 类 号：R273[医药卫生—中西医结合]