印度梨形孢诱导油菜抗旱性机理的初步研究  

Preliminary Study on Mechanisms of Drought Resistance in Brassica napus L.Conferred by Piriformospora indica

作　　者：陈佑源[1] 楼兵干[1] 高其康[2] 林福呈[1] 

机构地区：[1]浙江大学生物技术研究所,杭州310058 [2]浙江大学农生环测试中心,杭州310058

出　　处：《农业生物技术学报》2013年第3期272-281,共10页Journal of Agricultural Biotechnology

基　　金：浙江省自然科学基金(No.Y3100107;No.Z3080241和No.2009C13046)

摘　　要：印度梨形孢(Piriformospora indica)是一种根部内生真菌,能够促进许多植物的生长,提高作物的产量,而且还能诱导植物产生对生物或非生物胁迫的抗性。为了研究印度梨形孢对油菜(Brassica napus L.)抗旱性的影响,本研究用20%的聚乙二醇6000(PEG)对有印度梨形孢定殖和无印度梨形孢定殖的油菜植株进行模拟干旱胁迫处理,分析两者在受干旱胁迫后丙二醛(MDA)含量、相对电导率大小、脯氨酸(Pro)含量、抗氧化酶(超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT))活性以及干旱相关基因表达水平的差异。结果表明,接种印度梨形孢的油菜植株叶片中MDA含量和相对电导率均显著低于未接种印度梨形孢的油菜植株;Pro含量显著高于未接种印度梨形孢的油菜植株,在PEG处理后的第72小时,接种印度梨形孢的油菜叶片中Pro含量是未接种印度梨形孢的油菜的1.3倍;SOD、POD和CAT活性明显高于未接种印度梨形孢的油菜植株,PEG处理后的第24小时SOD、POD和CAT活性分别是对照油菜的1.17、1.38和1.27倍。RT-PCR分析表明,干旱胁迫下有印度梨形孢定殖的油菜叶片中编码合成脂质转运蛋白的基因575表达上调,PEG处理后的第9小时其表达量是对照的3.2倍。本研究研究结果表明印度梨形孢提高油菜对干旱胁迫的抗性与MDA含量、质膜透性、Pro含量、抗氧化酶活性和干旱相关基因的表达相关,印度梨形孢可能是通过提高油菜整体抗氧化能力、维持细胞生物膜完整性和细胞内渗透压以及降低膜脂的过氧化水平,从而增强了油菜对干旱胁迫的抗性。本研究初步明确了印度梨形孢提高油菜抗旱性的作用与部分机理,为深入研究印度梨形孢提高油菜的抗逆性作用及其机理提供基础资料。Piriformospora indica is a root endophytic fungus, which can promote the growth of many plants, enhance crop yield, and also confer biotic and abiotic stress tolerance to its host plants. In this study, in order to confirm the influence of P. indica on drought resistance of Brassica napus, the differences of malondialdehyde （MDA） content, the relative conductance, proline （Pro） content, antioxidant enzymes （superoxide dismutase （SOD）, peroxidase（POD） and catalase（CAT）） activity and expression level of drought-related genes between B.napus colonized and un-colonized by P. indica were analysed after treating by 20% polyethylene glycol-6000（PEG） solution. The results showed that the concentration of MDA and the relative conductance in the leaves of B. napus colonized by P. indica was significantly lower than that in un-colonized ones. The Pro content in the leaves of B. napus colonized by P. indica were significantly higher than that in un-colonized ones, and it was 1.3 folds of that after PEG treatment for 72 h. The activity of SOD, POD and CAT in the leaves ofB. napus colonized by P. indica were significantly higher than that in un-colonized plants, they were 1.17, 1.38 and 1.27 folds of the control ones after PEG treatment for 24 h, respectively. RT-PCR analysis showed that the expression of drought-related gene 575 which codes lipid-transfer protein was upregulated in leaves of B. napus colonized by P. indica, and the expression level was 3.2 folds o f control plants after PEG treatment for 9 h. This study indicated that the drought resistance improvement ofB. napus conferred by P. indica was related to MDA and Pro contents, plasmamembrane permeability, antioxidant enzymes activity and the expression level of drought-related genes. P. indica may enhance drought resistance of B. napus through improving total antioxidant ability, maintaining cell biomembrane integrity and cell osmotic pressure, and reducing the level of membrane lipid peroxidation of plants. This study preliminary confi

关 键 词：植物内生真菌 丙二醛 抗氧化酶 抗旱性 油菜 

分 类 号：S512.1[农业科学—作物学]