高、低脂系肉鸡肝脏脂肪合成代谢相关基因的表达差异分析  

Expression Analysis of Lipid Synthesis-related Genes in the Livers of Fat and Lean Broilers

作　　者：史铭欣[1] 史洪岩[1] 李辉[1] 王宁[1] 

机构地区：[1]东北农业大学动物科学技术学院,农业部鸡遗传育种重点实验室,哈尔滨150030

出　　处：《农业生物技术学报》2013年第3期306-312,共7页Journal of Agricultural Biotechnology

基　　金：国家重点基础研究发展规划(973)项目(No.2009CB941604);黑龙江省教育厅家禽遗传育种科技创新团队项目(No.2010td02)

摘　　要：为探讨高、低脂肉鸡肝脏脂肪合成代谢的差异,本研究以东北农业大学肉鸡高、低腹脂双向选择品系第14世代鸡群为实验材料,利用实时荧光定量PCR方法,比较了乙酰辅酶A羧化酶(ACC)、脂肪酸合成酶(FAS)、固醇调控元件结合蛋白1(SREBP1)、肝X受体(LXR)、甘油磷酸酰基转移酶(GPAT)、载脂蛋白A-1(ApoA-1)、葡萄糖转运蛋白2(GLUT2)和载脂蛋白B(ApoB)8个与脂肪合成代谢相关的基因在1～12周龄高、低脂系肉鸡肝脏中的表达模式和表达差异。结果显示,从总体上看,ACC、FAS、SREBP1、LXR、GPAT和ApoA-1基因在高脂系鸡肝脏中的表达量高于低脂系鸡的表达,其中,ACC、FAS和SREBP1基因在第10周龄高脂系鸡中的表达量均极显著高于低脂系鸡(P<0.01);LXR基因在第9和10周龄高脂系鸡中的表达量极显著高于低脂系鸡(P<0.01);ApoA-1基因在第6、8和10周龄高脂系鸡中的表达量极显著高于低脂系鸡(P<0.01),在第11周龄高脂系鸡中的表达量显著高于低脂系鸡(P<0.05);同时,研究发现,GLUT2基因在第5、8周龄低脂系鸡的表达量显著高于高脂系鸡(P<0.05),并且ApoB基因在7周龄低脂系鸡肝脏的表达量也显著性的高于高脂系鸡(P<0.05)。本研究表明,高、低脂系肉鸡肝脏脂肪合成相关基因的表达存在着明显的差异,高脂系肉鸡肝脏具有更强的脂肪合成能力。本研究结果为进一步揭示肉鸡脂肪性状形成的分子机制提供了基础研究资料。In order to investigate the differences of hepatic lipid synthesis between fat and lean lines of broiler chickens, the gene expression levels of acetyl CoA-carboxylase （A CC）, fatty acid synthase （FAS）, sterol regulatory element binding protein-1 （SREBP1）, liver X receptor （LXR）, glycerophosphate acyltransferase （GPA T）, apoprotein A-1 （ApoA -1）, glucose transporter （GLUT2） and apoprotein B （ApoB） genes were analyzed in livers of the 14th generation of Northeast Agricultural University broiler lines divergently selected for abdominal fat content （NEAUHLF） population using Real-time RT-PCR. The results revealed that, the expression levels ofA CC, FAS, SREBP1, LXR, GPA T and ApoA-1 genes, on the whole, were higher in fat line than that in lean line. In particular, the expression levels of A CC, FA S and SREBP1 genes were dramatically higher in fat line than that in lean line at the age of 10 weeks （P〈0.01）. In addition, the expression ofLXR gene was significantly higher in fat line than that in lean line at the age of 9 and 10 weeks （P〈0.01）, and the expression of ApoA -1 gene was significantly higher in fat line than that in lean line at the age of 6, 8, 10, and 1. 1 wk （P〈0.01 or P〈0.05）. However, the expression of GLUT2 gene was significantly higher in lean line than in fat line at the age of 5 and 8 weeks （P〈0.05）, and the expression ofApoB gene was also significantly higher in lean line than that in fat line at the age of 7 weeks （P〈0.05）. Taken together, these results suggested that fat broilers have higher a capacity for hepatic fat synthesis than that of lean broilers, which may lead to divergent phenotype for fatness traits. Our findings provide basic research material for further study on the molecular mechanisms of broiler fatness traits.

关 键 词：肉鸡 肝脏 脂肪合成 基因表达 

分 类 号：S852.2[农业科学—基础兽医学]