3，4-二硝基呋咱基氧化呋咱急性和亚慢性毒性研究  

3, 4 - dinitro-furazan-based oxidation furazan acute and subchronic toxicity studies

作　　者：孙苑菡[1] 杜文霞[1] 王玉玲[1] 张延巍[1] 邢亚飞[1] 谢锋[1] 

机构地区：[1]中国兵器工业集团第五二一研究所,西安710065

出　　处：《中华劳动卫生职业病杂志》2013年第3期217-220,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：中国兵器科学研究院预研课题（42001100202）

摘　　要：目的对3，4-二硝基呋咱基氧化呋咱（DNTF）的急性毒性和亚慢性毒性进行研究，确定DNTF的急性毒性分级、毒作用性质及主要靶器官，为制订职业接触限值提供依据。方法（1）急性毒性实验：采用经口灌胃法一次性染毒，观察大鼠的中毒症状，计算DNTF的LD50和95％可信限。（2）亚慢性实验：选用健康SD大鼠96只，随机分为25．0、56．2、125．0mg／kg染毒剂量组和阴性对照组，连续染毒90d，每周5d，每天1次。结果（1）急性经口毒性实验结果表明，DNTF大鼠经口LD50大于5000mg／kg，DNTF染毒小鼠经口LD504589mg／kg，95％可信限为4026～5230mg／kg，急性毒性分级属于低毒级化合物。（2）亚慢性毒性实验中，125、56．2mg／kg剂量组动物染毒后30min后出现精神萎靡被毛潮湿明显。125．0mg／kg染毒组12只大鼠出现腹泻、外阴分泌物增多，持续2-3d后恢复，但仍有大鼠染毒30min后被毛明显潮湿。高、中剂量雄性大鼠体重增长低于阴性对照组，差异有统计学意义（P〈0．05）。高、中、低剂量组雌性大鼠体重增长与对照组比较，差异无统计学意义（P〉0．05）。125mg／kg染毒组血清丙氨酸转氨酶、天冬氨酸转氨酶活力明显高于对照组，125mg／kg剂量组大鼠的肝、肾、肺、睾丸系数明显高于对照组，差异均有统计学意义（P〈0．05）。肝、肾、肺病理组织学检查可见明显的形态学改变。结论DNTF急性毒性分级属低毒级化合物，毒作用的靶器官为肝、肾、肺。Objective To study the 3, 4 - dinitro-furazan-based oxidation furazan （DNTF） of sub-acute toxicity and chronic toxicity, to determine the acute toxicity classification DNTF, the nature of toxic effects and major target organ for the development provide the basis for occupational exposure limits. Methods （1） Acute toxicity： The oral gavage method once infected, symptoms of poisoning of animals observed to calculate the LD50 DNTF and 95% confidence limits. （2） sub-chronic experiment： selection of 96 healthy SD rats were randomly divided into four groups, doses of 25, 56.2, 125 mg/kg and the negative control group, Exposure for ninety days, five days a week, once a day, The rats were killed at end of Exposure, heart, liver, spleen, lung, kidney, brain, testis, uterus were taken to observe the pathological changes. Results （1） Acute oral toxicity test results indicate that DNTF rat oral LDs0 greater than 5000 mg/kg, DNTF mice treated by oral LD50 4589 mg/kg, 95% confidence limit for the 4026~5230 mg/kg, Acute toxicity grade level is low toxicity compounds. （2） Sub-chronic toxicity experiment, the high-dose male rats, high, medium and low-dose group female rats weight gain than the negative control group, compared with the control group, the difference was statistically significant （P〈0.05）. 125 mg/kg of serum alanine aminotransferase, aspartate aminotransferase was significantly higher. 125 mg/kg dose groups, liver, kidney, lung, testicular factor was significantly higher. Liver, kidney, lung histological examination showed obvious morphological changes. Conclusion Acute toxicity grade DNTF low toxicity level compounds, target organ toxicity of liver, kidney and lung.

关 键 词：3 4-二硝基呋咱基氧化呋咱 毒性试验 急性 

分 类 号：R9[医药卫生—药学] R1