反义寡聚脱氧核糖核苷酸对猪促卵泡刺激素受体表达的影响  

Effect of Antisense Oligodeoxynucleotide on Expression of Porcine Follicle-stimulating Hormone Receptor.

作　　者：朱长虹[1] 熊忠明[1] 肖慧珠[1] 

机构地区：[1]同济医科大学计划生育研究所,武汉430030

出　　处：《中华男科学杂志》2000年第3期146-149,共4页National Journal of Andrology

基　　金：湖北省自然科学基金!项目 ( 99J15 8)

摘　　要：目的 :本研究旨在评价反义寡聚脱氧核糖核苷酸 (ODN)对促卵泡刺激素受体 (FSHR)mRNA表达的调节作用。　方法 :转染FSHR的CHO细胞系的mRNA水平用反转录 竞争性PCR方法进行定量测定。　结果 :结果显示用 10 μmol/L反义ODN可导致mRNA表达显著上升 ,2 4h后mRNA水平从 0 89± 0 0 6pg/ μg总RNA升至 1 6 4±0 0 8pg/ μg总RNA(P <0 0 5 )。用 0 33μmol/L反义ODN进行脂转染也得到相同结果 ,mRNA水平从 0 95± 0 0 8pg/ μg总RNA升高至 1 5 3± 0 0 7pg/ μg总RNA。这可能是由于FSHR蛋白翻译被抑制后上行调节mRNA合成所致 ,无义ODN对FSHRmRNA的表达没有任何作用。　结论 :本文结果提示针对猪FSHR翻译起始位点的反义ODN能上行调节猪FSHRmRNA的表达水平。Objectives: To assess the role of antisense oligodeoxynucleotide in regulating expression of FSHR gene Methods: Porcine follicle stimulating hormone receptor messenger ribonucleic acid was determined by reverse transcription competitive polymerase chain reaction Results: The results showed that direct culture with 10 μmol/L antisense ODN caused a paradoxical increase of mRNA from 0 89±0 06 pg/μg total RNA to 1 64±0 08 pg/μg total RNA ( P <0 05) Transfection with lipofectamine and 0 33 μmol/L antisense ODN caused an increase of mRNA from 0 95±0 08 pg/μg total RNA to 1 53±0 07pg/μg total RNA This was probably due to upregulation of mRNA synthesis resulting from inhibition of porcine FSHR protein translation The nonsense ODN had no effect on porcine FSHR mRNA Conclusions: These results indicate that an antisense ODN corresponding to the region of the translation start site of the porcine FSHR cDNA can upregulate the expression of porcine FSHR messenger ribonucleic acids

关 键 词：促卵泡刺激素受体 竞争性PCR ODN 表达 

分 类 号：R392[医药卫生—免疫学]