两株B型流感病毒的基因组序列分析  

Genome sequence analysis on two strains of influenza B virus

作　　者：岳秀芳[1,2] 夏咸柱[2] 于志君[2] 朱晓文[2] 刘红[2] 李硕[3] 范宝静[3] 王世申[3] 吴静[3] 武艳丽[3] 王铁成[2] 杨松涛[2] 黄耕[2] 高玉伟[2] 胡桂学[1] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118 [2]中国人民解放军军事医学科学院军事兽医研究所,吉林省人畜共患病预防与控制重点实验室,长春130062 [3]长春市疾病预防控制中心,长春130033

出　　处：《中国人兽共患病学报》2013年第3期267-273,共7页Chinese Journal of Zoonoses

基　　金：吉林省卫生厅科研课题(2009zc017)资助~~

摘　　要：目的了解两株B型流感病毒的基因组序列特征。方法对两株B型流感病毒进行全基因组序列测定和基因进化特性的分析。结果成功扩增出两株B型流感病毒的全基因组序列,并且两毒株的基因组均属于Yamagata系类似株Ⅱ系。两株病毒的基因组序列与疫苗株B/Florida/4/2006(Yamagata系)以及Victoria系代表株B/Brisbane/60/2008、B/Malay-sia/2506/2004相比,HA基因同源性为89.7%～97.3%,NB基因同源性则为95.4%～98.4%。两株病毒的HA蛋白与WHO推荐的疫苗株B/Florida/4/2006(Yamagata系)相比,存在11处氨基酸突变,其中HA的N131K氨基酸位点突变,可能对抗原性产生影响。NB的S198N氨基酸位点突变可能影响神经氨酸酶抑制剂的灵敏度。结论两株B型流感病毒的遗传进化状态稳定,但是与同分支中的毒株基因序列部分位点存在差异。To investigate genetic characterization of two influenza B virus strains, the complete genomes were sequenced and analyzed. Phylogenetic analysis of the whole genomes revealed that two strains were classified into the Yamagata-like clade. Comparison of genome sequences of strain CX1, CX2 and other vaccines （B/Florida/4/2006, B/Brisbane/60/2008, and B/Malaysia/2506/2004） showed that these influenza B viruses shared higher homology. The HA gene was 89.70/oo to 97.3% in homology, and NB gene was 95.4% to 98.4% in homology. However, phylogenetic analysis of these two strains with a WHO recommended vaccine strain （B/Florida/4/2006） showed that 11 variations in amino acid occurred on its HA protein. Mean- while, the N131K mutation in HA may impact its antigenicity, and the S198N mutation in NB may affect the sensitivity of NA inhibitor. These data indicated a close evolutionary relationship between these two influenza B virus strains despite minor varia- tions on certain genes of strains in the same clade.

关 键 词：B型流感病毒 基因序列 进化树 

分 类 号：R371.1[医药卫生—病原生物学]