一起肠产毒性大肠杆菌O128:H45引起的院内新生儿腹泻暴发  

An outbreak of nosocomial neonatal diarrhea caused by enterotoxigenic Escherichia coliO128:H45

作　　者：王红[1] 赵爱兰[2] 白向宁[2] 张正东[1] 许烈英[1] 崔敏莉 刘祥[1] 纪律[1] 叶长芸[2] 熊衍文[2] 李群[1] 

机构地区：[1]四川省自贡市疾病预防控制中心,自贡643000 [2]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206

出　　处：《中国人兽共患病学报》2013年第3期304-308,共5页Chinese Journal of Zoonoses

基　　金：国家"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项(No.2011ZX10004-001);传染病预防控制国家重点实验室开放课题"疑似单增李斯特菌感染患者的病原学监测研究"(No.2012SKLID304)资助~~

摘　　要：目的探讨一起院内新生儿腹泻暴发的病原及其特征。方法常规方法对可疑腹泻暴发住院新生儿患者粪便、医护人员肛拭子、婴儿奶粉和医院环境标本进行病原分离和鉴定,将分离到的病原菌进行毒力基因检测、药敏试验及PFGE和MLST分子分型分析。结果从12名住院新生儿腹泻患者黄色水样便和1份新生儿ICU病房配奶间的环境标本中分离出13株大肠杆菌O128∶H45和1株大肠杆菌O55,13株大肠杆菌O128∶H45耐热肠毒素基因st均为阳性、具有高度相似性的PFGE带型、MLST的型别均为ST2332型且具有相似的耐药谱。结论首次报道了由肠产毒性大肠杆菌O128∶H45引起的一起院内新生儿腹泻暴发。To recognize the etiology of nosocomial neonatal diarrhea, the pathogens from leces of diarrheal neonates, a nal swab of health care workers, and milk and environmental samples in NICU wards were screened. The results showed that Escherichia coli O128 ： H45 strains were isolated from 12 fecal and 1 environmental samples. All 13 isolates contained the heat stable enterotoxin gene st and showed the highly similar PFGE patterns, the same MLST sequence type （ST2332）, and the similar antibiotic resistance spectrum. It could be concluded that the neonatal diarrhea outbreak in hospitals was caused by enterotoxigenic Escherichia coli O128 ： H45.

关 键 词：肠产毒性大肠杆菌 大肠杆菌O128∶H45 毒力基因 PFGE MLST 

分 类 号：R378.2[医药卫生—病原生物学]