雷洛昔芬促进兔下颌骨骨折的愈合  

Raloxifene promotes the healing of mandibular fractures in rabbits

作　　者：刘凯[1] 石崇[1] 刘树发[1] 张洪凯[1] 

机构地区：[1]佳木斯大学附属第二医院,黑龙江省佳木斯市154007

出　　处：《中国组织工程研究》2013年第2期217-222,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:雷洛昔芬虽在临床中广泛应用于治疗骨质疏松,但对骨折愈合的影响尚未见报道。目的:观察雷洛昔芬对兔下颌骨骨折愈合的影响。方法:取新西兰大耳白兔24只,制备双侧下颌角1.5mm×10.0mm骨缺损模型,随机抽签法分为2组,实验组于造模后第2天开始给予7.5mg/(kgd)雷洛昔芬至30d,对照组不作处理。结果与结论:X射线观察造模后1周两组骨缺损区骨痂形成不明显。3周实验组缺损区模糊,对照组缺损区仍可见;4周实验组骨痂多,髓腔再通;对照组骨痂较多,髓腔未通。造模后1,3,4周实验组骨密度和血清骨源性碱性磷酸酶较对照组明显升高(P<0.05),均于第4周时达到高峰;造模后3,4周实验组骨痂中骨形态发生蛋白2的表达强度高于对照组(P<0.01)。提示雷洛昔芬能够促进成骨细胞分化,加速骨矿物沉积,同时诱导骨形态发生蛋白2表达从而加速骨折的愈合。BACKGROUND： Although raloxifene has been widely applied in the treatment of osteoporosis, its influence on fracture healing is unclear.OBJECTIVE： To investigate the effect of raloxifene on the healing of mandibular fractures in rabbits. METHODS： Totally 24 New Zealand white rabbits were collected. Model of bilateral mandibular angle bone defect （1.5 mm×10.0 mm） in rabbits was established. All rabbits were randomly divided into experimental and control groups. The rabbits in the experimental group were performed 7.5 mg/（kg？d） raloxifene at day 2 after modeling for 30 days, while those in the control group had no treatment.RESULTS AND CONCLUSION： X-ray observation showed that callus formation was not obvious in the two groups after modeling for 1 week. Defect area of the experimental group was indistinct, but that of the control group could be found at week 3. At week 4, callus in the experimental group was increased and medullary cavity passed again. Callus in the control group was more, and medullary cavity did not pass. Compared with control group, bone mineral density and serum bone alkaline phosphatase in the experimental group was increased obviously after modeling for 1, 3 and 4 weeks （P 〈 0.05）, and they both reached the peak. The expression of bone morphogenetic protein 2 in callus was higher than that of the control group after modeling for 3 and 4 weeks （P 〈 0.01）. These results suggest that raloxifene can promote osteoblast differentiation, accelerate bone mineral deposition, and induce bone morphogenetic protein 2 expression at the same time, and thereby accelerate the healing of bone fracture.

关 键 词：组织构建 骨组织构建 雷洛昔芬 下颌骨 骨折愈合 兔 骨形态发生蛋白 组织构建图片文章 

分 类 号：R318[医药卫生—生物医学工程]