芍药苷促椎间盘纤维环细胞增殖的作用  

Paeoniflorin accelerates annulus fibrosus cells proliferation

作　　者：陈少清[1,2] 林建平[1,3] 王诗忠[1,2] 洪钰[1] 陈乐春[2] 

机构地区：[1]福建中医药大学,福建省福州市350003 [2]教育部省部共建中医骨伤及运动康复重点实验室,福建省福州市350003 [3]福建省运动功能康复重点实验室,福建省福州市350003

出　　处：《中国组织工程研究》2013年第2期254-258,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(30973764);福建省卫生教育联合攻关计划项目(WKJ2008-2-48);福建省科技厅国际合作项目(2009I0010)~~

摘　　要：背景:芍药在颈椎病治疗中使用频率超过30%,其主要单体之一芍药苷具有抗炎、抗凋亡等作用,芍药苷对椎间盘纤维环细胞是否有保护作用,国内外未见报道。目的:探讨芍药苷对椎间盘纤维环细胞的增殖和保护作用。方法:采用酶消化法体外培养椎间盘纤维环细胞。取第3代纤维环细胞,培养过程中加入20μg/LFas配体诱导建立纤维环细胞凋亡模型,通过MTT法测定芍药苷干预纤维环细胞的最适宜浓度,AnnexinV/FITC-PI法观察芍药苷对椎间盘纤维环细胞的保护作用。结果与结论:芍药苷浓度20.8和2.08μmol/L对椎间盘纤维环细胞具有明显的促增殖作用。20μg/LFas配体能诱导细胞凋亡,20.8μmol/L芍药苷对Fas配体诱导椎间盘纤维环细胞有保护作用。BACKGROUND： Chinese herbaceous peony has been usually used in the treatment of cervical spondylosis in a frequency of over 30%. Paeoniflorin, known as one of the main monomers, has cell-protective abilities against inflammation and apoptosis. Despite these compelling observations, whether paeoniflorin protects annulus fibrosus cells is not reported.OBJECTIVE： To explore the proliferative and protective effects of paeoniflorin on intervertebral disc annulus fibrosus cells.METHODS： Annulus fibrosus cells derived from the intervertebral disc were digested with trypsin and collagenase type Ⅱ for the further culture in vitro. The third-passage annulus fibrosus cells were selected to establish an apoptosis model in response to Fas ligand （20 μg/L） and were interfered with paeoniflorin. The appropriate concentration of paeoniflorin was detected by the method of MTT. Apoptotic rate was evaluated with Annexin V/FITC double staining. RESULTS AND CONCLUSION： The 20.8 and 2.08 μmol/L paeoniflorin significantly promoted the proliferation of annulus fibrosus cells. The apoptotic rate of annulus fibrosus cells in vitro was significantly increased after induction with the 20 μg/L Fas ligand. 20.8 μmol/L paeoniflorin could protect annulus fibrosus cells against Fas ligand-induced apoptosis.

关 键 词：组织构建 脊柱组织构建 颈椎病 芍药苷 FASL 纤维环细胞 细胞凋亡 细胞增殖 细胞保护 椎间盘退变 国家自然科学基金 组织构建图片文章 

分 类 号：R318[医药卫生—生物医学工程]