肾虚血瘀型膝骨性关节炎大鼠模型的建立  

Establishment of a rat model of knee osteoarthritis with kidney deficiency and blood stagnation

作　　者：刘振峰 方锐 艾力江.阿斯拉 洪汉刚 邓迎杰 孟庆才 

机构地区：[1]新疆维吾尔自治区中医医院关节外科,新疆维吾尔自治区乌鲁木齐市830000

出　　处：《中国组织工程研究》2013年第2期270-274,共5页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区科技攻关项目(200933126)~~

摘　　要：背景:建立骨性关节炎的中医证候模型,有利于对其进行辨证施治研究。目的:采用病、证结合的方法建立肾虚血瘀型膝骨关节炎大鼠模型。方法:选择2月龄雌性SPF级SD大鼠30只,随机分为正常组、膝骨关节炎组和复合模型组,每组10只。采用经典的Hulth造模法建立膝骨关节炎模型,去卵巢法建立肾虚模型,肾上腺皮质激素和肾上腺素法建立血瘀模型。复合模型由上述3种模型复合而成,先建立肾虚模型,8周后同时进行血瘀模型与骨关节炎模型造模。结果与结论:复合模型组大鼠在肾虚模型建立后第3周时出现精神萎靡、活动不灵活、便溏等表现,在血瘀模型建立后第6周时大鼠出现皮毛无光泽、喜背光、舌质瘀紫、尾色及爪甲瘀青,体质量下降;低切还原黏度增高,血小板聚集性增强等血流缓慢、循环障碍特点;组织学染色观察发现膝骨关节炎组关节软骨结构有一定破坏但轻于复合模型组。可见实验成功建立了大鼠肾虚血瘀型大鼠膝骨关节炎模型,而肾虚血瘀加重了膝骨关节软骨的退变。BACKGROUND： To establish a model of osteoarthritis with traditional Chinese medicine syndromes is conductive to the syndrome differentiation studies.OBJECTIVE： To establish a rat model of knee osteoarthritis with kidney deficiency and blood stagnation.METHODS： A total of 30 female Sprague-Dawley rats aged 2 months, SPF grade, were randomly divided into normal group, knee osteoarthritis group and knee osteoarthritis with kidney deficiency and bloods stagnation group （combined group）, 10 rats in each group. Knee osteoarthritis models were established using Hulth method, kidney deficiency models were established using ovariectomized method, and blood stagnation models were established by injection of adrenal corticosteroids and epinephrine. Combined models were prepared based on the above-mentioned three models. Kidney deficiency models were prepared first followed by establishment of knee osteoarthritis and bloods stagnation models. RESULTS AND CONCLUSION： Rats in the combined group presented listlessness, non-flexible activities and loose stools at 3 weeks after establishment of kidney deficiency models, and coat lackluster, ptosis, obvious weight loss and blue tail at 6 weeks after establishment of bloods stagnation models. Rats in the combined group also showed that the reducing viscosity was significantly up-regulated and the platelet aggregation reinforced, with blood flow slow-moving, circulatory disorder and so on. Meanwhile, the articular cartilage of the knee osteoarthritis group showed cataplasia, but it was slighter compared with the combined group. The rat model of knee osteoarthritis with kidney deficiency and blood stagnation is established successfully, and kidney deficiency and blood stagnation can aggravate articular cartilage degeneration.

关 键 词：组织构建 组织构建实验造模 肾虚 血瘀 骨关节炎 模型 大鼠 省级基金 组织构建图片文章 

分 类 号：R318[医药卫生—生物医学工程]