创伤性深静脉血栓形成与血液中Hmox-1基因的表达  

Formation of traumatic deep venous thrombosis and mRNA expression of Hmox-1 in the blood

作　　者：郑燕科[1] 李宏坤[2] 汤善华[1] 赵敏[1] 吕仁发[1] 陈荣剑[1] 

机构地区：[1]解放军第184医院骨科,江西省鹰潭市335000 [2]昆明医学院第一附属医院骨科,云南省昆明市650032

出　　处：《中国组织工程研究》2013年第2期291-295,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:目前深静脉血栓的诊断主要依赖于影像学和实验室检查,其最致命的不足是血栓已经形成方能明确诊断。因此有必要采用先进的分子技术对其进行研究,探索其发生发展的内在规律。目的:观察Hmox-1在大鼠创伤性深静脉血栓形成过程中的变化,探讨其作为预测诊断深静脉血栓分子标记物的可行性。方法:采用钳夹大鼠双侧股静脉和双后肢人字石膏固定的方法建立创伤性深静脉血栓形成模型。依据造模时间和血栓形成状态,将100只实验大鼠随机分为5组:正常对照组、创伤即刻组、血栓形成前期组、血栓形成高峰期血栓形成组、血栓形成高峰期血栓不形成组。Realtime-PCR检测血液中Hmox-1基因表达,并切取双侧股静脉血管进行组织学观察血栓形成程度。结果与结论:建立大鼠创伤性深静脉血栓形成模型后25h,血栓形成率58%(38/65);血栓形成前期组、血栓形成高峰期血栓形成组Hmox-1表达高于正常对照组(P<0.05),而血栓形成高峰期血栓不形成组与正常对照组比较差异无显著性意义。Hmox-1在血液中的表达变化趋势与血栓形成生物学过程相符,有可能作为预测大鼠深静脉血栓形成的标志物。BACKGROUND： The diagnosis of deep vein thrombosis is mainly dependent on imaging and laboratory tests, and the most fatal lack is that thrombosis can be confirmed until it has been formed. Accordingly, it is necessary to explore inherent rules using molecular technology. OBJECTIVE： To observe the Hmox-1 expression in the process of formation of traumatic deep vein thrombosis in rats and to discuss the feasibility of Hmox-1 as a molecular marker for predictive diagnosis of deep vein thrombosis.METHODS： Bilateral femoral veins were clamped and both hind limbs were fixed in rats to prepare traumatic deep venous thrombosis models. According the modeling time and formation of thrombosis, 100 experimental rats were randomized into five groups： normal group, trauma group, pre-thrombosis group, group of thrombosis formation at peak stage, group of non-thrombosis formation at peak stage. Real time-PCR was used to detect blood Hmox-1 mRNA expression. Bilateral femoral veins were isolated for histological observation of thrombosis degree.RESULTS AND CONCLUSION： At 25 hours after modeling, the thrombosis rate was 58.46% （38∕65）. mRNA expression of Hmox-1 in the pre-thrombosis group and group of thrombosis formation at peak stage was higher than that of the normal group （P 〈 0.05）. There was no significant difference in the Hmox-1 mRNA expression between the normal group and the group of non-thrombosis formation at peak stage. These findings indicate that the variation tendency of Hmox-1 in the blood is consistent with the biological process of thrombosis formation, and Hmox-1 may become a marker for predictive diagnosis of deep venous thrombosis.

关 键 词：组织构建 组织构建细胞学实验 深静脉血栓 Hmox-1 预测诊断 大鼠 血栓形成 动物模型 组织构建图片文章 

分 类 号：R318[医药卫生—生物医学工程]