β-磷酸三钙/聚乳酸-聚羟基乙酸/异烟肼/左氧氟沙星缓释材料的成骨检测  

Osteogenesis detection for beta-tricalcium phosphate/poly(lactic-co-glycolic acid)/isoniazid/levofloxacin slow-release materials

作　　者：崔旭[1] 马远征[1] 李大伟[1] 薛海滨[1] 

机构地区：[1]解放军第309医院骨科,全军骨科中心,北京市100091

出　　处：《中国组织工程研究》2013年第3期381-386,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(81071454)~~

摘　　要：背景:在聚乳酸-聚羟基乙酸中加入β-磷酸三钙可调控其降解速率和强度。目的:观察β-磷酸三钙/聚乳酸-聚羟基乙酸/异烟肼/左氧氟沙星缓释材料修复兔股骨髁骨缺损的效果。方法:制作兔右股骨髁直径5mm、长10mm的圆柱形骨缺损模型,随机分3组,其中两组分别于骨缺损处植入β-磷酸三钙/聚乳酸-聚羟基乙酸材料和β-磷酸三钙/聚乳酸-聚羟基乙酸/异烟肼/左氧氟沙星缓释材料,空白对照组不植入任何材料。通过影像学、大体标本、组织学检查评价骨缺损修复效果。结果与结论:术后12周时,β-磷酸三钙/聚乳酸-聚羟基乙酸材料组和β-磷酸三钙/聚乳酸-聚羟基乙酸/异烟肼/左氧氟沙星缓释材料组骨缺损都得到修复,两组新骨占缺损区面积差异无显著性意义(P>0.05),空白对照组骨缺损未修复。表明β-磷酸三钙/聚乳酸-聚羟基乙酸/异烟肼/左氧氟沙星缓释材料可以很好修复兔股骨髁缺损。Abstract BACKGROUND： The degradation rate and strength of poly（lactic-co-glycolic acid） （PLGA） can be regulated by the addition of beta-tricalcium phosphate （β-TPC）.OBJECTIVE：To test the effect of β-TPC/PLGNisoniazid （INH）/levofloxacin （LVFX） composite on the repair of femoral condyle bone defects in rabbits.METHODS：Thirty New Zealand rabbits were used for preparing a rabbit model of bone defects with the diameter of 5 mm and the depth of 10 mm. The rabbits were randomly divided into three groups： group 1, group 2 and group 3. The former two groups were implanted with β-TPC/PLGA and I3-TPC/PLGA/INH/LVFX, respectively. The group 3 was untreated as the blank control group. The repair effects of each group were detected by imaging, gross specimen and histological examination.RESULTS AND CONCLUSION：At week 12 after treatment, bone defects in the two experimental groups were both radiographically repaired, and there was no significant difference between these two groups in the ratio of new bone area to bone defects area （P 〉 0.05）. The blank control group failed to restore bone defects. These results suggest that the PLGA/TCP/INH/LVFX material can effectively repair femoral condyle bone defects in rabbits.

关 键 词：生物材料 组织工程骨材料 聚乳酸-聚羟基乙酸 Β-磷酸三钙 骨缺损 骨结核 骨组织工程 国家自然科学基金 生物材料图片文章 

分 类 号：R318[医药卫生—生物医学工程]