丝素蛋白对纳米羟基磷灰石晶体生长的调控作用  

Effect of silk fibroin on the regulation of nano-hydroxyapatite crystal growth

作　　者：徐宁[1] 陈玉云[2] 钟建[2] 许国华[1] 叶晓健[1] 

机构地区：[1]解放军第二军医大学附属长征医院骨科,上海市200003 [2]纳米技术及应用国家工程研究中心,上海市200241

出　　处：《中国组织工程研究》2013年第3期418-418,418,共1页Chinese Journal of Tissue Engineering Research

基　　金：上海市科学与技术委员会基金资助项目(1052nm03201);国家重点基础研究发展计划项目(2009CB93000)~~

摘　　要：背景:前期研究表明丝素蛋白含量对丝素蛋白/纳米羟基磷灰石复合材料性能与结构有一定影响。目的:观察丝素蛋白含量对纳米羟基磷灰石晶体生长的调控作用。方法:采用物理化学方法制备出含0,10%,20%,30%,40%丝素蛋白的丝素蛋白/纳米羟基磷灰石复合物,通过热重分析仪、傅里叶红外光谱仪、透射电镜、X射线粉末衍射仪分析丝素蛋白含量对纳米羟基磷灰石晶体生长的调控作用。结果与结论:丝素蛋白的加入对纳米羟基磷灰石晶体的成核和生长具有明显调控作用:在纳米羟基磷灰石晶粒沿c轴高度择优生长,长径比增大,形貌由短棒状调控为针状。丝素蛋白含量对纳米羟基磷灰石晶体的成核和生长影响微弱,但对晶粒在丝素蛋白基质的有序排布有明显的调控作用:当丝素含量为10%和20%时,纳米羟基磷灰石晶粒呈放射状有序排布;丝素含量为30%和40%时,纳米羟基磷灰石晶粒无序团聚,说明30%含量是丝素蛋白与纳米羟基磷灰石的饱和吸附临界点。BACKGROUND： Silk fibroin （SF） content has been reported to have great influences on the properties and structure of SF/nano-hydroxyapatite （SF/n-HA） complex in previous studies.OBJECTIVE： To observe the effect of SF content on the crystal growth of n-HA.METHODS： SF/n-HA complex was prepared using the method of physical chemistry. The effect of SF content （0, 10%, 20%, 30%, 40%） on the crystal growth of n-HA was studied using thermogravimetric analysis, Fourier transform infrared spectroscopy, transmission electron microscope, and X-ray powder diffractometer.RESULTS AND CONCLUSION： The results show that SF affected the crystal nucleation and growth of n-HA obviously. SF induced n-HA crystals to preferentially grow along the c axis, the n-HA aspect ratio increased, and n-HA morphology changed from rod-like to needle-like. When SF content was 10% and 20%, n-HA crystals radically dispersed in SF matrix ordedy; when SF content was 30% and 40 %, the n-HA crystals were disordered in SF matrix.These findings indicate that 30% SF is the critical binding percent in the SF/n-HA preparation.

关 键 词：纳米羟基磷灰石 调控作用 晶体生长 丝素蛋白 羟基磷灰石复合材料 胶原纤维 生物矿化过程 化学成分 

分 类 号：R318.08[医药卫生—生物医学工程]