新型纳米支架与神经干细胞的凋亡  

New-type nano-scaffolds and neural stem cell apoptosis

作　　者：周继辉[1,2] 姚猛[3] 王岩松[3] 刘玉刚[3] 隋福革[1,2] 赵丛然[1,2] 田飞鹏[1,2] 何晓峰[1,2] 

机构地区：[1]大庆龙南医院 [2]齐齐哈尔医学院附属第五医院,黑龙江省大庆市163453 [3]哈尔滨医科大学附属第二医院脊柱外科,黑龙江省哈尔滨市150086

出　　处：《中国组织工程研究》2013年第3期419-426,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(30800260);黑龙江省自然科学基金(D200916;D200902)~~

摘　　要：背景:胶原作为脊髓组织工程的良好支架有利于神经细胞及神经纤维的黏附和生长,但机械性能较差,需要在制备时提高其基本性能,还应对种子细胞的生物学行为产生积极影响。目的:分析胶原纳米组织工程支架的性能,检测其对神经干细胞凋亡行为及相关基因表达的影响。方法:采用电纺丝技术制备纤维定向排列及非定向排列的胶原纳米纤维膜,并对其进行表征。将新生SD大鼠脊髓源性神经干细胞分别与纤维定向排列及非定向排列的胶原纳米纤维膜共培养,并设置单独神经干细胞培养为对照,检测细胞凋亡情况及相关基因表达变化。结果与结论:定向及非定向胶原纳米纤维膜的直径及形貌均达到纳米组织工程支架标准,溶胀系数较高,孔隙率较高,力学性能佳。与对照组比较,两胶原纳米纤维膜组神经干细胞凋亡率明显降低(P<0.05),细胞凋亡相关基因中bcl-2基因表达量明显增加,bax及Caspase-3基因表达明显下降;两胶原纳米纤维膜组神经干细胞凋亡率差异无显著性意义。表明新型胶原纳米组织工程支架性能良好,能够抑制细胞凋亡,从基因表达水平调节神经干细胞的凋亡行为。BACKGROUND： As a good material for spinal cord tissue engineering scaffold, collagen is conducive to adhesion and growth of nerve cells and nerve fibers. But it needs to be improved in the preparation process because of its poor mechanical properties, not only to improve its basic performance, but also to have a positive impact on the biological behavior of the seed cells.OBJECTIVE： To observe the properties of new nano-scaffolds for tissue engineering and to detect the changes of neural stem cell apoptosis and related gene expression affected by the nano-scaffold.METHODS： Aligned and randomly oriented nanofibrous scaffolds were made of collagen by using electrospinning technology. Superficial morphous, porosity, mechanical property, swelling coefficient,degradation disposition were tested. Spinal cord derived neural progenitor cells were cultured and identified, and then the cells were cultured on aligned and randomly oriented collagen nanofibrous scaffolds. Cells cultured under normal conditions served as control group. The changes of neural stem cell apoptosis and related gene expression were tested.RESULTS AND CONCLUSION： Superficial morphous of electrospun aligned and randomly oriented collagen nanofibrous scaffolds were in accordance with contrivable requisition, their porosities were supernal, mechanical properties Wecul retured on alignfiedRe＇ swelling coefficients were satisfactory. Compared witha thend randomly oriented collagen nanofibrous scaffolds decreascoed significantly （P〈0.05）,expression level of apoptosis-related gene bcl-2 significantly increased, bax and Caspase-3 significantly decreased. There was no significant difference between the calls on aligned and randomly oriented collagen nanofibrous scaffolds.These findings indicate that properties and histocompatibility of new nano-scaffolds for tissue engineering are satisfactory, which can inhibit and regulate cell apoptosis from the level of gene expression.

关 键 词：生物材料 纳米生物材料 胶原 纳米支架 纤维膜 组织工程支架 增殖 凋亡 分化 基因 神经干细胞 定向排列 国家自然科学基金 生物材料图片文章 

分 类 号：R318[医药卫生—生物医学工程]