Sequence Analysis of Type III Effector tccP and tccP2 Genes in Escherichia coli O157:H7 from Chinese Water-chestnut  

大肠杆菌O157:H7 CWN11Ⅲ效应因子tccP和tccP2基因序列分析(英文)

作　　者：张雪寒[1] 叶青[1] 刘亚栋 何孔旺[1] 

机构地区：[1]江苏省农业科学院兽医研究所/农业部兽用生物制品工程技术重点实验室/国家兽用生物制品工程技术研究中心,江苏南京210014 [2]南京天邦生物科技有限公司,江苏南京21000

出　　处：《Agricultural Science & Technology》2013年第2期202-205,共4页农业科学与技术（英文版）

基　　金：Supported by the National Key Technology Research and Development Program of China (2012BAK08B07);the National Natural Science Foundation of China (31201919)~~

摘　　要：[Objective] This study aimed to analyze the type III effector tccP and tccP2 genes in Escherichia coli O157：H7 from Chinese water-chestnut. [Method] Gene-specific and locus-specific primers were utilized to amplify tccP/tccP2 and their flanking regions for sequence analysis. [Result] E. coli O157：H7 CWN11 harbored intact tccP and tccP2 genes, however, the number of proline-rich repeats in tccP gene was only one that probably resulted in biological incapability, whereas, the tccP2 gene consisted of five and half proline-rich repeats and could encode functional protein. [Conclusion] Here, we reported the first sequence of tccP gene that consisted of only one proline-rich repeat and tccP2 was assumed to play a crucial role in colonization and subsequent signaling cascades.[目的]分析大肠杆菌O157:H7CWN11效应因子tccP和tccP2基因序列。[方法]PCR扩增tccP和tccP2完整基因,以及含有tccP和tccP2侧翼序列的基因,测序分析。[结果]CWN11同时含有tccP和tccP2两个基因,前者含有一个富含脯氨酸的重复序列,而tccP2含有5.5个脯氨酸重复序列,并且第28位密码子无缺失,非假基因。参考菌株SakaitccP含有4.5个脯氨酸重复序列,而tccP2为假基因。[结论]首次报道非典型大肠杆菌O157:H7CWN11同时含有完整的tccP和tccP2,并且tccP可能编码无功能蛋白,而tccP2在细菌定植和激发信号级联中发挥替代功能。

关 键 词：EHEC O157：H7 Chinese water-chestnut tccP gene tccP2 gene proline-rich repeats 

分 类 号：S852.61[农业科学—基础兽医学]