Determination and Comparison of Pigment Contents in Leaves of Punica granatum L. Hybrids with Different Leaf Colors  

不同叶色石榴杂种单株叶片色素含量的比较(英文)

作　　者：郭晓丽[1] 李洪涛 李东伟 张丽婷[1] 吴亚君[1] 陈延惠[1] 

机构地区：[1]河南农业大学园艺学院,河南郑州450002 [2]郑州市园林科学研究所,河南郑州450003

出　　处：《Agricultural Science & Technology》2013年第2期243-247,共5页农业科学与技术（英文版）

基　　金：Supported by Science and Technology Project of He'nan Province(072102150001);Science and Technology Project of Zhengzhou City(083SGYG24123-6)~~

摘　　要：[Objective] This study aimed to investigate the reason for the different leaf colors of pomegranate hybrid progeny. [Method] Spectrophotometry was used to determine the contents of three pigments, including chlorophyll, carotenoid and anthocyanin, in the leaves of eight pomegranate individuals and their parents at growth stage. [Result] The results showed that during the early growth stage, the anthocyanin contents of four pomegranate individuals with red leaves were higher than the control, to be specific, the anthocyanin content of No.5 pomegranate individual reached the highest of 4.12 mg/g; the chlorophyll and carotenoid contents of four pomegranate individuals with yellow-green leaves showed consistent dynamic trends with the control, to be specific, the chlorophyll content and chlorophyll/carotenoid ratio of No.4 pomegranate individual reached the highest, while the chlorophyll a/carotenoid b ratio of No.3 pomegranate individual was the lowest. During vigorous growth stage, the contents of chlorophyll, carotenoid and anthocyanin of eight pomegranate individuals showed consistent dynamic trends, with no significant difference with the control. Analysis of variance indicated that there were significant differences in the contents of chlorophyll and carotenoid among different pomegranate individuals at different growth stages and in the content of anthocyanin among different pomegranate individuals, while no significant difference was observed in the chlorophyll/carotenoid ratio among different pomegranate individuals at different growth stages. [Conclusion] The differences in leaf colors among pomegranate hybrid progeny are caused by different contents and ratios of anthocyanin and chlorophyll at growth stage.[目的]石榴杂种后代不同叶片颜色的成因。[方法]采用分光光度法对8个石榴杂种单株和2个亲本对照单株生长期叶片中叶绿素、类胡萝卜素和花青素的含量进行了研究。[结果]在生长初期,叶片表现红色的4个单株花青素含量均高于对照,其中5号单株花青素含量最高,达到4.12mg/g;叶片表现黄绿色的4个单株叶绿素与类胡萝卜素的含量变化趋势与对照基本一致,其中4号单株叶绿素的含量和叶绿素/类胡萝卜素的值最高,而3号单株叶绿素a/叶绿素b的值最低。在生长旺盛期,8个石榴单株叶绿素、类胡萝卜素和花青素含量的变化趋势一致,与对照无明显差异。经方差分析,不同石榴单株在不同的生长期其叶绿素和类胡萝卜素含量均存在显著差异,不同石榴单株间花青素含量存在显著差异,而不同石榴单株在不同的生长期其叶绿素/类胡萝卜素的值不存在显著差异。[结论]石榴杂种后代间叶片颜色不同是由于生长期花青素、叶绿素含量和比值不同造成的。

关 键 词：Pomegranate （Punica granatum L） Individual hybrid CHLOROPHYLL CAROTENOID ANTHOCYANIN 

分 类 号：S665.4[农业科学—果树学]