β防御素2对大鼠肺部炎症细胞因子的影响  

The Impact of rBD2 on the Lung Inflammatory Cytokines of Rat

作　　者：沈桢巍[1] 方路[2] 姜敏敏[3] 刘显东[1] 雷撼[4] 

机构地区：[1]同济大学附属东方医院重症监护室,上海200120 [2]同济大学附属东方医院肾脏内科,上海200120 [3]上海市杨浦区中心医院呼吸内科,上海200090 [4]同济大学附属东方医院呼吸内科,上海200120

出　　处：《成都医学院学报》2013年第1期17-20,共4页Journal of Chengdu Medical College

基　　金：国家自然科学基金面上项目(NO:30670932);浦东新区卫生局面上项目(PW2005A-10);浦东新区卫生局面上项目(PWRd2007-17)

摘　　要：目的探讨β防御素2(rBD2)对大鼠肺部炎症反应的影响及其可能机制。方法构建大鼠rBD2的cDNA慢病毒载体和RNA干扰载体,双向调节rBD2在大鼠肺组织内的表达水平,ELISA法检测肺组织中IL-1α、IL-1β、IL-4、IL-10的表达变化,Western blot检测肺组织中rBD2的表达,Real time PCR法检测肺组织中rBD2mRNA的表达。结果成功构建rBD2cDNA慢病毒和RNA干扰载体,并成功转染。大鼠肺部感染时,上调rBD2可降低IL-1α、IL-1β等促炎细胞因子的水平,提高抗炎细胞因子IL-4、IL-10的含量(P<0.01)。结论 rBD2可调节大鼠肺组织炎症相关细胞因子的水平,参与并抑制大鼠肺部炎症反应。Objective To study the impact of rat beta defensin-2（rBD2） on the pulmonary inflammation of rat and the possible mechanism. Methods To establish rat rBD2 cDNA lentiviral vector and vector for rBD2 siRNA. Both upregulation and downregulation of rBD2 expression were conducted to examine its effect on the pulmonary inflammation of rat. ELISA assay was conducted to evaluate the expression of cytokines,including IL-1α, IL-1β, IL-4 and IL-10. The expression of rBD2 in the lung was detected by Western blot; the expression of rBD2 mRNA was detected with Real-time PCR. Results Overexpression and RNAi lentiviral recombinant vectors of rBD2 were successfully constructed. Up regulation of rBD2 expression could decrease the level of IL-1α, IL-1β and increase the level of IL-4,IL-10（P（0.01）. Conclusion rBD2 could regulate the expression of cytokines and play a role as an anti-inflammatory role in the development of pulmonary inflammation in rat.

关 键 词：大鼠 β防御素2 细胞因子 

分 类 号：R563.1[医药卫生—呼吸系统]