肉桂与黄连不同配伍比例对交泰丸中小檗碱含量的影响  

Effects of Cinnamon and Coptis in Different Compatibility Proportion on Content of Berberine in Jiaotai Bolus

作　　者：陈广[1] 陆付耳[2] 李伶俐[3] 徐丽君[2] 邹欣[2] 王开富[2] 董慧[2] 黄召谊[1] 胡亚耘[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合科,武汉430030 [2]华中科技大学同济医学院附属同济医院中西医结合研究所,武汉430030 [3]华中科技大学同济医学院附属普爱医院中医科,武汉430033

出　　处：《医药导报》2013年第3期353-355,共3页Herald of Medicine

基　　金：国家自然科学基金资助项目(30801492)

摘　　要：目的探讨黄连与肉桂不同配伍比例对交泰丸中小檗碱溶出率的影响。方法各组中药采取水煎法,小檗碱检测条件:色谱柱XTerraP18(4.6 mm×250 mm,5μm);流动相:0.05 mol.L-1磷酸二氢钾(用磷酸调pH至3.5)-乙腈(70∶30);检测波长:346 nm;柱温:40℃;流速0.9 mL.min-1;进样量:10μL。结果黄连∶肉桂=1∶0.25,1∶0.5与黄连单煎剂小檗碱提取率差异明显,且随肉桂比例提高,小檗碱煎出率降低。结论肉桂对黄连有效成分小檗碱的溶出具一定的抑制作用。Objective To explore the effects of cinnamon and coptis in different compatibility proportion on the dissolu- tion rate of berberine from fiaotai bolus. Methods All the drugs were decocted and berberine content was determined on HPLC with a UV detector based on the following parameters： XTerra P18 （4.6 mm×250 mm, 5 μm） column, acetonitrile- 0.05 mmol · L-1 potassium dihydrogenphosphate （30 ： 70, pH 3.5 adjusted by phosphoric acid） as mobile phase, detection wavelength 346 nm, column temperature 40 ℃, flow rate 0.9 mL · min-1 and loading sample volume 10 μL. Results There was marked difference in the extraction ratio of berberine between cinnamon combined with coptis in the proportion of 1 ： 0.25, 1 ： 0.5, and coptis single decoction. Moreover, the decoction rate of berberine decreased with cinnamon proportion increasing. Conclusion Cinnamon has inhibitory effect on dissolution of berberine from coptis.

关 键 词：黄连 肉桂 小檗碱 溶出率 色谱法 高效液相 

分 类 号：R286[医药卫生—中药学] R283[医药卫生—中医学]