复方“前愈”总黄酮部位的分离纯化与含量测定  

Separation,Purification and Determination of Total Flavonoids from Compound Qianyu

作　　者：孟军华 李居怡 吴金虎 陈永刚 邹吉利 何阳 

机构地区：[1]武汉市第三医院药学部,430060

出　　处：《医药导报》2013年第3期359-362,共4页Herald of Medicine

基　　金：武汉市科技局学科带头人计划项目(201150530155);武汉市卫生局基金资助项目(WZ11C07)

摘　　要：目的从中药复方"前愈"中提取分离总黄酮化学部位,建立总黄酮及淫羊藿苷的含量测定方法。方法采用溶剂法结合聚酰胺树脂柱层析法分离纯化总黄酮部位;应用紫外分光光度法和高效液相色谱法,分别测定分离提取液及纯化液中总黄酮及淫羊藿苷的含量。结果溶剂法提取分离所得总黄酮含量8.86%,淫羊藿苷含量为0.14%;纯化后总黄酮含量为28.36%,淫羊藿苷含量为3.19%。结论该分离纯化方法操作简便合理,可显著提高前愈复方中总黄酮及淫羊藿苷含量。Objective To extract and separate total flavonoids from the Chinese herbal compound qianyu and establish a method for determination of total flavonoids and icariin. Methods The solvent combined with the polyamide resin column chromatography was adopted to separate and purify the total flavonoids. The content of total flavonoids and icariin in the extracts was determined by ultraviolet spectrophotometry and HPLC. Results The contents of total flavonoids and icariine were 8.86% and 0.14% after extraction,and were 28.36% and 3.19% after purification, respectively. Conclusion This extrac- tion and purification approach is simple and reasonable, which can evidently improve the content of total flavonoids and icariin extracted from compound qianyu.

关 键 词：前愈 总黄酮部位 淫羊藿苷 分离纯化 含量测定 

分 类 号：R286[医药卫生—中药学] R284[医药卫生—中医学]