Immobilization of nitrite oxidizing bacteria using biopolymeric chitosan media  

作　　者：Pranee Lertsutthiwong Duangcheewan Boonpuak Wiboonluk Pungrasmi Sorawit Powtongsook 

机构地区：[1]Metallurgy and Materials Science Research Institute,Chulalongkorn University [2]Department of Environmental Engineering,Faculty of Engineering,Chulalongkorn University [3]National Center for Genetic Engineering and Biotechnology [4]Center of Excellence for Marine Biotechnology,Department of Marine Science,Chulalongkorn University

出　　处：《Journal of Environmental Sciences》2013年第2期262-267,共6页环境科学学报（英文版）

基　　金：supported by the Ratchadaphiseksomphot Endowment Fund

摘　　要：The effects of chitosan characteristics including the degree of deacetylation, molecular weight, particle size, pH pretreatment and immobilization time on the immobilization of nitrite-oxidizing bacteria （NOB） on biopolymeric chitosan were investigated. Nitrite removal efficiency of immobilized NOB depended on the degree of deacetylation, particle size, pH pretreatment on the surface of chitosan and immobilization time. Scanning electron microscope characterization illustrated that the number of NOB cells attached to the surface of chitosan increased with an increment of immobilization time. The optimal condition for NOB immobilization on chitosan was achieved during a 24-hr immobilization period using chitosan with the degree of deacetylation larger than 80% and various particle size ranges between 1-5 mm at pH 6.5. In general, the NOB immobilized on chitosan flakes has a high potential to remove excess nitrite from wastewater and aquaculture systems.The effects of chitosan characteristics including the degree of deacetylation, molecular weight, particle size, pH pretreatment and immobilization time on the immobilization of nitrite-oxidizing bacteria （NOB） on biopolymeric chitosan were investigated. Nitrite removal efficiency of immobilized NOB depended on the degree of deacetylation, particle size, pH pretreatment on the surface of chitosan and immobilization time. Scanning electron microscope characterization illustrated that the number of NOB cells attached to the surface of chitosan increased with an increment of immobilization time. The optimal condition for NOB immobilization on chitosan was achieved during a 24-hr immobilization period using chitosan with the degree of deacetylation larger than 80% and various particle size ranges between 1-5 mm at pH 6.5. In general, the NOB immobilized on chitosan flakes has a high potential to remove excess nitrite from wastewater and aquaculture systems.

关 键 词：chitosan immobilization nitrite-oxidizing bacteria pH adjustment 

分 类 号：X703[环境科学与工程—环境工程]