Isolation and characterization of an enoyl-acyl carrier protein reductase gene from microalga Isochrysis galbana  

作　　者：郑明刚 梁科鹏 王波 孙修勤 岳燕燕 万文文 郑立 

机构地区：[1]Research Center for Marine Ecology,First Institute of Oceanography,State Oceanic Administration [2]College of Life Science,Shangdong Agricultural University [3]Food College of Shihezi University

出　　处：《Chinese Journal of Oceanology and Limnology》2013年第2期398-406,共9页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.41106148);the Ocean Public Welfare Scientific Research Project(Nos.GHME2001SW02,200905019,200805039);the Science and Technology Development Program of Shandong Province(No.2011GHY11533)

摘　　要：In most bacteria, plants and algae, fatty acid biosynthesis is catalyzed by a group of freely dissociable proteins known as the type II fatty acid synthase （FAS II） system. In the FAS II system, enoyl- acyl carrier protein reductase （ENR） acts as a determinant for completing the cycles of fatty acid elongation. In this study, the cDNA sequence of ENR, designated as IgENR, was isolated from the microalga lsochrysis galbana CCMM5001. RACE （rapid amplification of cDNA ends） was used to isolate the full-length cDNA oflgENR （1 503 bp）, which contains an open reading frame （ORF） of 1 044 bp and encodes a protein of 347 amino acids. The genomic DNA sequence oflgENR is interrupted by four introns. The putative amino acid sequence is homologous to the ENRs of seed plants and algae, and they contain common coenzyme- binding sites and active site motifs. Under different stress conditions, real-time quantitative polymerase chain reaction （RT-qPCR） showed the expression oflgENR was upregulated by high temperature （35℃）, and downregulated by depleted nitrogen （0 mol/L）. To clarify the mechanism of lipids accumulating lipids, other genes involved in lipids accumulation should be studied.In most bacteria,plants and algae,fatty acid biosynthesis is catalyzed by a group of freely dissociable proteins known as the type II fatty acid synthase(FAS II) system.In the FAS II system,enoylacyl carrier protein reductase(ENR) acts as a determinant for completing the cycles of fatty acid elongation.In this study,the cDNA sequence of ENR,designated as IgENR,was isolated from the microalga Isochrysis galbana CCMM5001.RACE(rapid amplification of cDNA ends) was used to isolate the full-length cDNA of IgENR(1 503 bp),which contains an open reading frame(ORF) of 1 044 bp and encodes a protein of 347 amino acids.The genomic DNA sequence of IgENR is interrupted by four introns.The putative amino acid sequence is homologous to the ENRs of seed plants and algae,and they contain common coenzymebinding sites and active site motifs.Under different stress conditions,real-time quantitative polymerase chain reaction(RT-qPCR) showed the expression of IgENR was upregulated by high temperature(35℃),and downregulated by depleted nitrogen(0 mol/L).To clarify the mechanism of lipids accumulating lipids,other genes involved in lipids accumulation should be studied.

关 键 词：lsochrysis galbana enoyl-ACP reductase real-time quantitative PCR TEMPERATURE NITROGEN 

分 类 号：Q943[生物学—植物学]