PROTEIN ADSORPTION AND CELL ADHESION ON RGD-FUNCTIONALIZED SILICON SUBSTRATE SURFACES  

作　　者：Wei-fang Tong Xiao-li Liu Fei Pan 武照强 Wen-wen Jiang 

机构地区：[1]Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application,Department of Polymer Science and Engineering,College of Chemistry,Chemical Engineering and Materials Science,Soochow University [2]College of Chemistry and Material Sciences,South-Central University for Nationalities

出　　处：《Chinese Journal of Polymer Science》2013年第3期495-502,共8页高分子科学（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (Nos.20974122,21174098);the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)

摘　　要：A method was developed to modify silicon surfaces with good protein resistance and specific cell attachment. A silicon surface was initially deposited using a block copolymer of N-vinylpyrrolidone （NVP） and 2-hydroxyethyl methacrylate （HEMA） （PVP-b-PHEMA） film through surface-initiated atom transfer radical polymerization and then further immobilized using a short arginine-glycine-aspartate （RGD） peptide. Our results demonstrate that the RGD-modified surfaces （Si-RGD） can suppress non-specific adsorption of proteins and induce the adhesion of L929 cells. The Si-RGD surface exhibited higher cell proliferation rates than the unmodified silicon surface. This research established a simple method for the fabrication of dual-functional silicon surface that combines antifouling and cell attachment promotion.A method was developed to modify silicon surfaces with good protein resistance and specific cell attachment. A silicon surface was initially deposited using a block copolymer of N-vinylpyrrolidone （NVP） and 2-hydroxyethyl methacrylate （HEMA） （PVP-b-PHEMA） film through surface-initiated atom transfer radical polymerization and then further immobilized using a short arginine-glycine-aspartate （RGD） peptide. Our results demonstrate that the RGD-modified surfaces （Si-RGD） can suppress non-specific adsorption of proteins and induce the adhesion of L929 cells. The Si-RGD surface exhibited higher cell proliferation rates than the unmodified silicon surface. This research established a simple method for the fabrication of dual-functional silicon surface that combines antifouling and cell attachment promotion.

关 键 词：Cell adhesion Protein adsorption RGD Poly（N-vinylpyrrolidone） ATRP. 

分 类 号：O647.3[理学—物理化学]