组胺H_2受体阻滞剂对免疫介导小鼠再生障碍性贫血发生的阻断作用  

The development of immune-mediated aplastic anemia in mice can be blocked by cimetidine

作　　者：汪明春[1] 廖继东[2] 赵忻[2] 林晨[3] 江振友[3] 马秀君[2] 

机构地区：[1]深圳市血液病研究所,深圳市红十字会医院血液科,518035 [2]暨南大学医学院中心实验室 [3]暨南大学医学院微生物教研室

出　　处：《中华血液学杂志》2000年第10期527-529,共3页Chinese Journal of Hematology

基　　金：广东省卫生厅科学研究基金!资助项目 (A19972 6 1);广州市科委科学研究基金!资助项目 (97 Z 6 6 0 5 )

摘　　要：目的　探讨组胺H2 受体阻滞剂对免疫介导小鼠再生障碍性贫血 (再障 )的发生有无阻断作用。方法　通过给全身亚致死量照射的BALB/c小鼠转输H 2相同、MLS不同的DBA/ 2小鼠胸腺、淋巴结混合细胞诱导小鼠再障发生 ,实验组每天腹腔注射 1次甲氰咪胍 ,共 8次 ,于照射后第 14天 ,观察股骨骨髓增生程度。结果　腹腔注射 0 .5 0 ,1.0 0 ,2 .0 0mg·(10g体重 ) -1·d-1甲氰咪胍的小鼠的股骨平均骨髓造血组织容量分别为 (4 6 .5 8± 2 0 .41) %、(5 1.88± 2 4.94) %和 (5 9.12± 32 .48) % ,显著高于再障组的 (19.99± 10 .98) % (P <0 .0 1) ,而与单纯照射组 (5 3 .81± 31.72 ) %相比 ,差异无显著性 (P >0 .0 5 )。结论　组胺H2 受体阻滞剂能阻断半相合淋巴细胞诱导经亚致死量照射的小鼠的再障的发生。Objective To investigate the effect of H 2 receptor antagonist cimetidine on the development of immune-mediated aplastic anemia(AA) in mice. Methods The immune-mediated aplastic anemia mice model was induced by irradiation and half-matched allogeneic lymphocytes transfusion(ALT). The mice were divided into 3 groups: the irradiation alone control group, the irradiation+ALT group (AA group) and the irradiation+ALT+cimetidine group. In cimetidine group, each mouse was intraperitoneally injected cimetidine at different doses once a day for 8 days. On day 14, the bone marrow histology was examined. Results In 0.5*!mg, 1.0*!mg and 2.0 mg·(10g) -1 ·d -1 cimetidine groups, the percentages of hematopoietic tissue volume in bone marrow were (46.58±20 41)%, (51.88±24.94)% and (59.12±32.48)%, respectively, and all much higher than that in AA group [(19.99±10.98)%, P<0.01], but were not different from that in irradiated control group[(53.81±31 72)%, P>0.05]. Conclusion The development of immune-mediated aplastic anemia in irradiated mice by half-matched allogeneic lymphocytes transfusion was blocked by H 2 receptor antagonist cimetidine.

关 键 词：再生障碍性贫血 组胺H2受体阻滞剂 免疫介导 

分 类 号：R556.5[医药卫生—血液循环系统疾病]