黄芪总皂甙对病毒性心肌炎小鼠慢性期SERCA活性的影响  

Changes of SERCA activity in chronic viral myocarditis mice treated with astragaloside

作　　者：陈相健[1] 陆曙[1] 耿茜 张寄南 马文珠 

机构地区：[1]江苏省南京医科大学第一附属医院心研所,210029

出　　处：《江苏医药》2000年第10期757-759,共3页Jiangsu Medical Journal

基　　金：国家"九五"攻关基金!(96 90 60213);江苏省科委社会发展基金!(BS970 2 8与BS980 39);江苏省卫生厅项目!(H970 1)

摘　　要：目的　探讨黄芪提取物 -黄芪总皂甙对柯萨奇病毒B3(CVB3)感染的病毒性心肌炎(VMC)小鼠慢性期心肌肌浆网钙泵 (SERCA)活性变化的影响。方法　将BALB/C小鼠分为正常组(31只 )、模型组 (36只 )及黄芪组 (2 6只 ) ,模型组及黄芪组每只小鼠以 2× 10 4 TCID50 CVB3(TCID50 为10 -5 83)腹腔注射感染。黄芪组每鼠每天腹腔注射 0 15ml黄芪总皂甙注射液 ,正常及模型组予同体积的生理盐水。 3个月后 ,观察各组小鼠死亡率及心肌病理改变 ,检测血清心肌肌钙蛋白I(cTnI)含量及心肌组织中SERCA活性。结果　 (1)和正常组、黄芪组相比 ,模型组小鼠死亡率显著增高 (P <0 0 5～ 0 0 0 1)。 (2 )感染 3个月后模型组、黄芪组心肌肌钙蛋白I(cTnI)及心肌病理改变与正常组无显著性差异 (P >0 0 5 )。 (3)模型组心肌SERCA活性显著低于正常组及黄芪组 (P <0 0 1)。结论　黄芪总皂甙可以降低VMC小鼠的死亡率 。Objective To investigate the changes of cardiac Sarco/Endoplasmic Ca 2+ ATPase(SERCA) activity in chronic viral myocarditis(VMC) mice treated with Astragaloside. Methods The BALB/C mice were randomly divided into three groups:normal group(N),model group (M) and Astragaloside group (A).M group and A group were infected by 2×10 4TCID 50 CVB 3(TCID 50 10 -5 83 )intraperitoneally.The mortality and cardiac pathological changes were observed after 3 months.The serum cTnI and cardiac SERCA activity were measured meanwhile.Results (1)Compared with N group and A group,The mortality of M group was significantly higher ( P< 0 05～0 001).(2)The cTnI and cardiac pathological changes of M and A groups had no difference with those of N group ( P> 0 05).(3)The cardiac SERCA activity of V group were significantly lower than those of N group and A group( P< 0 01).Conclusion Astragaloside could decrease the mortality of viral myocarditis and reverse the down regulations of the SERCA activity in wice with chronic mice.

关 键 词：黄芪总皂甙 柯萨奇病毒B3 病毒性心肌炎 SERCA 

分 类 号：R542.21[医药卫生—心血管疾病]