手足口病肠道病毒检测中两种核酸提取方法的比较  

Comparison of two extraction methods for viral nucleic acid in hand foot-mouth disease

作　　者：洪巧珍 崔大伟[2] 杨先知[2] 秦志梅[2] 谢国良[2] 陈瑜[2] 

机构地区：[1]浙江省衢州市柯城区人民医院检验科,浙江衢州324000 [2]浙江大学医学院附属第一医院检验科,浙江杭州310003

出　　处：《中国现代医生》2013年第4期111-112,117,共3页China Modern Doctor

基　　金："十二五"国家科技重大专项(2012ZX10004-210)

摘　　要：目的对手工核酸提取法和全自动核酸提取仪法在手足口肠道病毒RNA荧光定量PCR检测中提取效率、重复性、耗时和成本进行比较。方法手工法试剂盒和全自动核酸提取仪对样本进行核酸提取,采用实时荧光定量PCR进行评价。结果提取仪法阳性46例,试剂盒法阳性43例,二者无明显差异(P>0.05)。提取仪法重复性高于试剂盒法(CV3.5%<5%)。核酸液终体积:提取仪法70μL,试剂盒法50μL。提取仪法耗时较短,成本较高;试剂盒法耗时较长,成本较低。结论两种方法结果无显著差异,均能满足临床需要,核酸提取仪法更具优势。Objective To compare the relative extraction efficiency, repeatability, time consumption and cost between manual nucleic acid extraction and automated nucleic acid extraction system using in analyze hand-foot-mouth enterovirus infection. Methods Nucleic acid templates were extracted from samples using nucleic acid extraction and automated nucleic acid extraction system methods. Nucleic acid was tested for RNA quantity by real-time fluorescent quantitative PCR assay. Results Automated nucleic acid extraction system had 46 positive samples and 43 positive samples by manual kit. There was no significant difference （P 〉 0.05）. Repeatability for automated nucleic acid extraction system was better than manual kit （CV3.5%〈5%）. Manual kit had 50 μL nucleic acid extraction final volume, however,70 μL in automated nucleic acid extraction system. Time consume was shorter, but cost was high with automated nucleic acid extraction system; Time consume was longer, but cost was lower with manual kit. Conclusion There is no significant difference in these two nucleic acid extraction methods. They can meet clinical needs, automated nucleic acid extraction system is equips with some advantages.

关 键 词：手足口病 病毒核酸提取 CT值 

分 类 号：R450[医药卫生—治疗学]